Details

Autor(en) / Beteiligte

• Dalla Rosa, Luciana
• Da Silva, Aleksandro S.
• Ruchel, Jader B.
• Gressler, Lucas T.
• Oliveira, Camila B.
• França, Raqueli T.
• Lopes, Sonia T.A.
• Leal, Daniela B.R.
• Monteiro, Silvia G.

Titel

Influence of treatment with 3′-deoxyadenosine associated deoxycoformycin on hematological parameters and activity of adenosine deaminase in infected mice with Trypanosoma evansi

Ist Teil von

• Experimental parasitology, 2013-10, Vol.135 (2), p.357-362

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• The treatment influence in activity of adenosine deaminase in plasma and brain of infected mice with Trypanosoma evansi. •The treatment in the doses used caused no alterations in hematological parameters.•The plasma total protein levels can be reduced as a consequence of this treatment.•The activity of ADA had inhibition in plasma of animals treated with pentostatin.•The activity of ADA had inhibition in brain of animals treated with pentostatin. This study aimed to verify the effect of 3′-deoxyadenosine and deoxycoformycin on hematologic parameters and adenosine deaminase (ADA) activity in plasma and brain of mice infected with Trypanosoma evansi. Seventy animals were divided into seven groups, which were divided into two subgroups each for sampling on days 4 and 8 post-infection (PI). The groups were composed of three uninfected groups (A–C), namely, not-treated (A), treated with 3′-deoxyadenosine (B), and treated with deoxycoformycin (C) and four infected groups, mice with T. evansi (D–G), namely, not-treated (D), treated with 3′-deoxyadenosine (E), treated with deoxycoformycin (F), and treated with a combination 3′-deoxyadenosine and deoxycoformycin (G). Hematological parameters and ADA activity were evaluated in plasma and brain. Animals in groups B and C exhibited a reduction in the levels of plasma total protein compared group A. Animals in groups D and F showed changes in the hematological parameters. The ADA activity significantly reduced in the animals of groups C, D, F and G. Mice in the group E presented increased ADA activity in plasma. Therefore, we conclude that the treatment interferes significantly in the hematologic parameters in mice infected with T. evansi. On the other hand, when the ADA inhibitor was used we observed a significant decrease in the values of hematocrit, total erythrocytes, and hemoglobin concentration. The deoxycoformycin was able to inhibit the ADA activity of parasite thus it may be one of the mechanisms of efficacy of this treatment.