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Diagnosis of hand, foot, and mouth disease caused by EV71 and other enteroviruses by a one-step, single tube, duplex RT-PCR
Journal of medical virology, 2012-11, Vol.84 (11), p.1803-1808
Jiang, Bingfu
Zhang, Jianhua
You, Xianhui
Dong, Chen
Cheng, Xianfeng
Dai, Xing
Meng, Jihong
2012
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Jiang, Bingfu
Zhang, Jianhua
You, Xianhui
Dong, Chen
Cheng, Xianfeng
Dai, Xing
Meng, Jihong
Titel
Diagnosis of hand, foot, and mouth disease caused by EV71 and other enteroviruses by a one-step, single tube, duplex RT-PCR
Ist Teil von
Journal of medical virology, 2012-11, Vol.84 (11), p.1803-1808
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2012
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
Hand, foot, and mouth disease (HFMD) is caused mainly by enterovirus 71 (EV71) and other enteroviruses (EVs) such as Coxsackie A16 in China. EV71 infection can lead to severe clinical manifestations and even death. Other EVs, however, generally cause mild symptoms. Thus, early and accurate distinction of EV71 from other EVs for HFMD will offer significant benefits. A one‐step, single tube, duplex RT‐PCR assay is described in the present study to detect simultaneously EV71 and other EVs. The primers used for the duplex RT‐PCR underwent screening and optimization. The detection threshold was 0.001 TCID50/ml for EV71 and 0.01 TCID50/ml for other EVs. The positive rate of enterovirus detection in 165 clinical samples reached 68.5%, including 46.1% for EV71 and 22.4% for other EVs. Of all the severe HFMD cases, EV71 was responsible for 85.3% cases. The positive rate of EV71 fell markedly by day 8 after onset. In addition, sequencing of EV71 specific amplicons from duplex RT‐PCR revealed that C4a was the predominant subgenotype of EV71 circulating in Nanjing, China. The accuracy and reliability of the assay suggest strongly that the one‐step, single tube, duplex RT‐PCR will be useful for early diagnosis and monitoring of EV71 and other EV infections. J. Med. Virol. 84:1803–1808, 2012. © 2012 Wiley Periodicals, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0146-6615
eISSN: 1096-9071
DOI: 10.1002/jmv.23391
Titel-ID: cdi_proquest_miscellaneous_1443377280
Format
–
Schlagworte
Animals
,
Biological and medical sciences
,
China
,
Diagnostic tests
,
DNA Primers - genetics
,
Enterovirus
,
Enterovirus A, Human - classification
,
Enterovirus A, Human - genetics
,
Enterovirus A, Human - isolation & purification
,
epidemiological surveillance
,
Foot-and-mouth disease
,
Fundamental and applied biological sciences. Psychology
,
Genotype
,
Hand, Foot and Mouth Disease - diagnosis
,
Human viral diseases
,
Humans
,
infectious disease
,
Infectious diseases
,
Medical sciences
,
Microbiology
,
Miscellaneous
,
Molecular Diagnostic Techniques - methods
,
molecular dignosis
,
Molecular Sequence Data
,
Multiplex Polymerase Chain Reaction - methods
,
Polymerase chain reaction
,
Prevalence
,
Reverse Transcriptase Polymerase Chain Reaction - methods
,
RNA, Viral - genetics
,
Sensitivity and Specificity
,
Sequence Analysis, DNA
,
Viral diseases
,
Viral infections
,
Virology
,
Virology - methods
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