UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 8 von 21
Datensatz exportieren als...
BibTeX
Hepatitis E virus isolated from rabbits is genetically heterogeneous but with very similar antigenicity to human HEV
Journal of medical virology, 2013-04, Vol.85 (4), p.627-635
Wang, Song
Dong, Chen
Dai, Xing
Cheng, Xianfeng
Liang, Jiuhong
Dong, Min
Purdy, Michael A.
Meng, Jihong
2013
Details
Autor(en) / Beteiligte
Wang, Song
Dong, Chen
Dai, Xing
Cheng, Xianfeng
Liang, Jiuhong
Dong, Min
Purdy, Michael A.
Meng, Jihong
Titel
Hepatitis E virus isolated from rabbits is genetically heterogeneous but with very similar antigenicity to human HEV
Ist Teil von
Journal of medical virology, 2013-04, Vol.85 (4), p.627-635
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2013
Link zum Volltext
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
Rabbit hepatitis E virus (HEV) in China may represent a novel HEV genotype, although no consensus has been reached. It is unclear whether the ORF2 capsid protein containing the immunodominant epitopes from rabbit HEV differs from those of human HEV. In this study, 661 bile samples collected from domestic rabbits in Jiangsu province, eastern China were amplified by RT‐nPCR using a set of HEV universal ORF2 primers. All 42 (6.4%) positive PCR products were sequenced. Phylogenetic analysis using the ORF2 sequences of 557 bp in length showed the Jiangsu isolates were separate from HEV genotypes 1, 2, 3, 4, avian HEV and rat HEV, and clustered together with rabbit HEV sequences. These 42 isolates were divided into five branches including two newly identified in the present study. Comparison with rabbit HEV sequences from China available in GenBank, using a 298 bp ORF2 segment, showed these sequences clustered together into a unique rabbit HEV clade, and were divided into eight sub‐branches with high genetic heterogeneity. In addition, 267 serum samples collected from domestic rabbits, serial serum samples from two rhesus monkeys experimentally infected with HEV genotype 1 or 4, and serial serum samples from two New‐Zealand rabbits infected experimentally with rabbit HEV were tested simultaneously by EIA using recombinant truncated ORF2 capsid proteins derived from rabbit and human HEV. The virtually identical results obtained suggest that rabbit and human HEV ORF2 antigens contain very similar immunodominant epitopes. All these data are helpful to identify the biological characteristics of the newly identified rabbit HEV. J. Med. Virol. 85:627–635, 2013. © 2013 Wiley Periodicals, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0146-6615
eISSN: 1096-9071
DOI: 10.1002/jmv.23504
Titel-ID: cdi_proquest_miscellaneous_1443368598
Format
–
Schlagworte
Animals
,
Antigenicity
,
Biological and medical sciences
,
China
,
Cluster Analysis
,
Epitopes - immunology
,
Fundamental and applied biological sciences. Psychology
,
Genotype
,
Genotype & phenotype
,
Hepatitis
,
Hepatitis Antibodies - blood
,
Hepatitis E - veterinary
,
Hepatitis E - virology
,
Hepatitis E virus
,
Hepatitis E virus - classification
,
Hepatitis E virus - genetics
,
Hepatitis E virus - immunology
,
Hepatitis E virus - isolation & purification
,
Human viral diseases
,
Humans
,
Infectious diseases
,
Macaca mulatta
,
Medical sciences
,
Microbiology
,
Miscellaneous
,
Molecular Sequence Data
,
Phylogenetics
,
Phylogeny
,
Proteins
,
rabbit
,
Rabbits
,
Reverse Transcriptase Polymerase Chain Reaction
,
RNA, Viral - genetics
,
Rodent Diseases - virology
,
Sequence Analysis, DNA
,
Serotyping
,
Viral diseases
,
Viral Proteins - genetics
,
Viral Proteins - immunology
,
Virology
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX