Details

Autor(en) / Beteiligte

• Huang, Wenbin, MD
• Williamson, Sean R., MD
• Rao, Qiu, MD
• Lopez-Beltran, Antonio, MD
• Montironi, Rodolfo, MD
• Eble, John N., MD
• Grignon, David J., MD
• Idrees, Muhammad T., MD
• Emerson, Robert E., MD
• Zhou, Xiao-jun, MD
• Zhang, Shaobo, MD
• Baldridge, Lee Ann, BA, HT(ASCP)
• Hahn, Noah M., MD
• Wang, Mingsheng, MD
• Koch, Michael O., MD
• Cheng, Liang, MD

Titel

Novel markers of squamous differentiation in the urinary bladder

Ist Teil von

• Human pathology, 2013-10, Vol.44 (10), p.1989-1997

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Summary Urinary bladder squamous cell carcinoma and urothelial carcinoma with squamous differentiation are often high-grade and high-stage tumors that are thought to be associated with a poorer prognosis and response to therapy compared with urothelial carcinoma without divergent differentiation. Therefore, recognition of a squamous component is increasingly important in guiding prognosis and therapy. We investigated the expression of MAC387, desmoglein-3, and TRIM29 in pure squamous cell carcinoma and urothelial carcinoma with squamous differentiation to determine whether they have utility as diagnostic biomarkers for squamous differentiation. Eighty-four cases were retrieved from participating institutions including 51 pure urinary bladder squamous cell carcinomas and 33 urothelial carcinomas with squamous differentiation. MAC387, desmoglein-3, and TRIM29 antibodies demonstrated positive staining in pure squamous cell carcinoma in 51 (100%), 46 (90%), and 48 (93%) cases, respectively. Urothelial carcinoma with squamous differentiation showed reactivity for MAC387, desmoglein-3, and TRIM29 in the squamous component for 32 (97%), 26 (79%), and 32 (97%) cases, respectively. MAC387 demonstrated a sensitivity of 99% and a specificity of 70% for squamous differentiation, whereas desmoglein-3 yielded a sensitivity of 86% and a specificity of 91%. No urothelial component showed greater than 10% labeling for desmoglein-3. TRIM29 labeling showed a sensitivity of 95%, but a poorer specificity of 33%. In summary, MAC387 and desmoglein-3 are reliable diagnostic markers for supporting the morphologic impression of squamous differentiation in urinary bladder carcinoma. Desmoglein-3 shows high specificity, whereas TRIM29 was most likely to demonstrate labeling in areas without light microscopically recognizable squamous differentiation.