Journal of invertebrate pathology, 1986, Vol.47 (1), p.21-31
1986
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Details
- Autor(en) / Beteiligte
- Titel
- Effects of Bacillus sphaericus 1593 and 2362 spore/crystal toxin on cultured mosquito cells
- Ist Teil von
- Journal of invertebrate pathology, 1986, Vol.47 (1), p.21-31
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 1986
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- An in vitro assay system for the toxin of Bacillus sphaericus strains 1593 and 2362 has been developed utilizing cultured Culex quinquefasciatus cells. The cytotoxic activity of extracts of B. sphaericus strain 1593 did not necessarily correlate with insecticidal activity. Cytotoxicity and larvicidal activity were neutralized by immune rabbit serum prepared against crude toxin extracts as well as by serum prepared against purified toxin from strain 2362. This purified toxin was also found to be cytotoxic. Activation with mosquito larval gut homogenates enhanced cytotoxicity of both 1593 extracts and purified toxin from 2362. The activity of cytotoxic preparations against three mosquito cell lines paralleled the activity of B. sphaericus spores against larvae of these mosquito species. The results suggest the presence of a protoxin and one or more cytotoxic proteins derived from it.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-2011eISSN: 1096-0805DOI: 10.1016/0022-2011(86)90159-XTitel-ID: cdi_proquest_miscellaneous_14355414
- Format
- –
- Schlagworte
- AEDES, AEDES AEGYPTI, AEDES ALBOPICTUS, Animals, AUXILIAIRE DE LUTTE BIOLOGIQUE, BACILLUS, Bacillus - pathogenicity, Bacillus sphaericus, Bacterial Proteins - toxicity, Bacterial Toxins - toxicity, BIOLOGICAL CONTROL ORGANISMS, cell cultures, Cell Survival - drug effects, Cells, Cultured, CULEX, CULEX QUINQUEFASCIATUS, Culicidae, Culicidae - microbiology, Endotoxins, Hemolysin Proteins, mode of action, mosquito, ORGANISMOS PARA CONTROL BIOLOGICO, spore crystals, toxin, TOXINAS, TOXINE, TOXINS