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Inositol phosphates compete with nucleic acids for binding to bovine leukemia virus matrix protein: Implications for deltaretroviral assembly
Proteins, structure, function, and bioinformatics, 2013-08, Vol.81 (8), p.1377-1385
Qualley, Dominic F.
Lackey, Crystal M.
Paterson, Justin P.
2013
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Qualley, Dominic F.
Lackey, Crystal M.
Paterson, Justin P.
Titel
Inositol phosphates compete with nucleic acids for binding to bovine leukemia virus matrix protein: Implications for deltaretroviral assembly
Ist Teil von
Proteins, structure, function, and bioinformatics, 2013-08, Vol.81 (8), p.1377-1385
Ort / Verlag
United States: Blackwell Publishing Ltd
Erscheinungsjahr
2013
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
ABSTRACT The matrix (MA) domain of retroviral Gag proteins plays a crucial role in virion assembly. In human immunodeficiency virus type 1 (HIV‐1), a lentivirus, the presence of phosphatidylinositol‐(4,5)‐bisphosphate triggers a conformational change allowing the MA domain to bind the plasma membrane (PM). In this study, the MA protein from bovine leukemia virus (BLV) was used to investigate the mechanism of viral Gag binding to the membrane during replication of a deltaretrovirus. Fluorescence spectroscopy was used to measure the binding affinity of MA for two RNA constructs derived from the BLV genome as well as for single‐stranded DNA (ssDNA). The importance of electrostatic interactions and the ability of inositol hexakisphosphate (IP6) to compete with nucleic acids for binding to MA were also investigated. Our data show that IP6 effectively competes with RNA and DNA for BLV MA binding, while [NaCl] of greater than 100 mM is required to produce any observable effect on DNA‐MA binding. These results suggest that BLV assembly may be highly dependent on the specific interaction of the MA domain with components of the PM, as observed previously with HIV‐1. The mode of MA binding to nucleic acids and the implications for BLV assembly are discussed. Proteins 2013; 81:1377–1385. © 2013 Wiley Periodicals, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0887-3585
eISSN: 1097-0134
DOI: 10.1002/prot.24281
Titel-ID: cdi_proquest_miscellaneous_1434018516
Format
–
Schlagworte
Animals
,
Bioinformatics
,
Bovine leukemia virus
,
Cattle
,
Enzootic Bovine Leukosis - microbiology
,
fluorescence spectroscopy
,
Gene Products, gag - metabolism
,
inositol phosphates
,
Inositol Phosphates - metabolism
,
Leukemia Virus, Bovine - physiology
,
nucleic acid-binding proteins
,
Nucleic Acids - metabolism
,
retroviral Gag
,
retrovirus assembly
,
RNA packaging
,
Spectrometry, Fluorescence
,
Virus Replication
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