Details

Autor(en) / Beteiligte

• Subirós-Funosas, Ramon
• Nieto-Rodriguez, Lidia
• Jensen, Knud J.
• Albericio, Fernando

Titel

COMU: scope and limitations of the latest innovation in peptide acyl transfer reagents

Ist Teil von

• Journal of peptide science, 2013-07, Vol.19 (7), p.408-414

Ort / Verlag

England: Blackwell Publishing Ltd

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• The methodology for peptide bond formation is undergoing a continuous evolution where the main actors are being renewed. In recent years, coupling reagents based on the Oxyma scaffold, such as the uronium salt COMU, has been a groundbreaking contribution to the field. The advantages of COMU over classic benzotriazole‐based reagents (HATU, HBTU, HCTU, TBTU) were proven in terms of solubility and coupling efficiency in bulky junctions in our groups and others. However, some aspects of the use of COMU need to be revised and improved, such as the stability of commercial samples in organic solvents, which hampers the compatibility with long synthesis in automated synthesizers. In this review, an overview of the main features and suggestions to improve the use of COMU are presented, along with a discussion on the best conditions for its use in microwave‐assisted peptide robots. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd. Nowadays there is a continuous renovation in the methodology for peptide bond formation. COMU as uronium salt derivate from Oxyma scaffold has improved the coupling efficiency in comparison with classic benzotriazole‐based reagents (HATU, HBTU, HCTU, TBTU). Nevertheless, there is still room of improvement in the performance of COMU, in example the stability of commercial batches in solution, which hampers the compatibility with automated synthesis.