Details

Autor(en) / Beteiligte

• Wang, Ming
• Li, Chenglong
• Yu, Beiqin
• Su, Liping
• Li, Jianfang
• Ju, Jingfang
• Yu, Yingyan
• Gu, Qinlong
• Zhu, Zhenggang
• Liu, Bingya

Titel

Overexpressed miR-301a promotes cell proliferation and invasion by targeting RUNX3 in gastric cancer

Ist Teil von

• Journal of gastroenterology, 2013-09, Vol.48 (9), p.1023-1033

Ort / Verlag

Tokyo: Springer Japan

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Background MicroRNAs can promote or suppress the evolution of malignant behaviors by regulating multiple targets. We aimed to determine the expression of miR-301a recently screened in gastric cancer, to investigate the biological effects of miR-301a and to identify the specific miR-301a target gene. Methods Quantitative real-time RT-PCR was used to test miR-301a expression. Functional effects were explored by a water-soluble tetrazolium salt assay, a colony formation assay in soft agar, a migration assay, an invasion assay and cytometry used to determine apoptosis and cell cycle. Nude mice were inoculated subcutaneously by retrovirus-mediated stably expressed SGC-7901 cells. The target gene was determined by bioinformatic algorithms, dual luciferase reporter assay and Western blot. Results Firstly, we found that miR-301a was significantly upregulated both in cells and tissues of gastric cancer. The expression level of miR-301a was inversely correlated with tumor differentiation of gastric cancer tissues. Secondly, miR-301a promoted cell growth, soft agar clonogenicity, migration, invasion, and decreased cell apoptosis induced by cisplatin in vitro, while blockage of miR-301a reduced the percentage of G2/M phase cells via flow cytometry in gastric cancer cells. Ectopic expression of miR-301a enhanced the subcutaneous tumorigenesis in vivo. Finally, miR-301a directly downregulated RUNX3 expression post-transcriptionally in gastric cancer. Conclusion Our results demonstrate that miR-301a plays important roles in the development of gastric cancer.