Details

Autor(en) / Beteiligte

• Akter, Rashida
• Kyun Rhee, Choong
• Rahman, Md. Aminur

Titel

A stable and sensitive voltammetric immunosensor based on a new non-enzymatic label

Ist Teil von

• Biosensors & bioelectronics, 2013-12, Vol.50, p.118-124

Ort / Verlag

Kidlington: Elsevier B.V

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• A stable and sensitive electrochemical carcinoembryonic antigen immunosensor based on a new non-enzymatic label (3, 3′, 5, 5′-tetramethylbenzidine (TMB)) was fabricated using gold nanoparticles-deposited polydopamine film (PD/AuNPs) as an anti-CEA antibody immobilizing platform. TMB was conjugated with CEA and utilized as an electrochemical label. The PD/AuNPs platform provides enhanced conductivity, thus the redox reaction of TMB label was observed at the immunosensor probe. The immunosensor probe was characterized using scanning electron microscopy and electrochemical impedance spectroscopy. The CEA detection was based on the competitive binding of free- and TMB-conjugated-CEA to the anti-CEA antibody. Using differential pulse voltammetry, CEA can be selectively detected as low as 10±0.6pg/mL. The present immunosensor method was applied to CEA-spiked human serum samples and the recovery results were satisfactory. ●A simple and sensitive CEA immunosensor was fabricated using a nonenzymatic label.●3, 3′, 5, 5′-tetramethylbenzidine (TMB) was used as the electrochemical label.●The TMB based CEA immunosensor had a low detection limit and wide linear range.