Molecular cell, 2013-06, Vol.50 (5), p.749-761
2013
Details
- Autor(en) / Beteiligte
- Titel
- A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway
- Ist Teil von
- Molecular cell, 2013-06, Vol.50 (5), p.749-761
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2013
- Link zum Volltext
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- The Drosophila piRNA pathway provides an RNA-based immune system that defends the germline genome against selfish genetic elements. Two interrelated branches of the piRNA system exist: somatic cells that support oogenesis only employ Piwi, whereas germ cells utilize a more elaborate pathway centered on the three gonad-specific Argonaute proteins (Piwi, Aubergine, and Argonaute 3). While several key factors of each branch have been identified, our current knowledge is insufficient to explain the complex workings of the piRNA machinery. Here, we report a reverse genetic screen spanning the ovarian transcriptome in an attempt to uncover the full repertoire of genes required for piRNA-mediated transposon silencing in the female germline. Our screen reveals key factors of piRNA-mediated transposon silencing, including the piRNA biogenesis factors CG2183 (GASZ) and Deadlock. Our data uncover a previously unanticipated set of factors preferentially required for repression of different transposon types. •Proper transposon silencing in Drosophila germ cells depends on at least 74 genes•Screening identifies piRNA biogenesis factors and effector proteins•Silencing of distinct transposons requires different mechanisms
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/j.molcel.2013.04.007Titel-ID: cdi_proquest_miscellaneous_1430850858
- Format
- –
- Schlagworte
- animal ovaries, Animals, Argonaute Proteins - genetics, Argonaute Proteins - metabolism, biogenesis, Data processing, DNA Transposable Elements, Drosophila, Drosophila melanogaster - genetics, Drosophila Proteins - genetics, Drosophila Proteins - metabolism, Female, females, Gene Expression Profiling - methods, Gene Silencing, genes, germ cells, immune system, Microtubule-Associated Proteins - genetics, Microtubule-Associated Proteins - metabolism, oogenesis, Ovary - physiology, Peptide Initiation Factors - genetics, Peptide Initiation Factors - metabolism, proteins, Reproducibility of Results, RNA Interference, RNA, Small Interfering - genetics, RNA, Small Interfering - metabolism, somatic cells, transcriptome, transposons