Details

Autor(en) / Beteiligte

• Bowers, William D
• Delbert, Sherri Sue
• Hunter, Richard L
• McIver, Robert T

Titel

Fragmentation of oligopeptide ions using ultraviolet laser radiation and Fourier transform mass spectrometry

Ist Teil von

• Journal of the American Chemical Society, 1984-11, Vol.106 (23), p.7288-7289

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

1984

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The effective use of pulsed lasers to fragment ions in mass spectrometers has proven to be difficult. One problem is that with quadrupole and magnetic sector mass spectrometers the interaction region between the laser and the ion beam is very small. Even with a 1-nA ion beam current fewer than 1000 ions are irradiated during the 20-ns pulse of an excimer laser. Another difficulty is that a complete mass spectrum cannot be obtained for each laser pulse because the scan speed is far slower than the duration of the laser pulse. These limitations are alleviated in ion cyclotron resonance spectrometers (ICR) because the ions are stored in an analyzer cell for up to several seconds and can be irradiated with many laser pulses. In this paper the authors demonstrate that oligopeptide ions stored in an ICR analyzer cell are fragmented efficiently by ultraviolet laser radiation. In addition, a complete mass spectrum of the photofragment ions is obtained for each pulse of the laser by Fourier transform detection.