Details

Autor(en) / Beteiligte

• Parks, Randi J.
• Howlett, Susan E.

Titel

H-89 decreases the gain of excitation–contraction coupling and attenuates calcium sparks in the absence of beta-adrenergic stimulation

Ist Teil von

• European journal of pharmacology, 2012-09, Vol.691 (1-3), p.163-172

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• This study used the selective protein kinase A (PKA) inhibitor H-89 (N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide) to determine the role of basal PKA activity in modulating cardiac excitation–contraction coupling in the absence of β-adrenergic stimulation. Basal intracellular cyclic AMP (cAMP) levels measured in isolated murine ventricular myocytes with an enzyme immunoassay were increased upon adenylyl cyclase activation (forskolin; 1 and 10μM) or phosphodiesterase inhibition (3-isobutyl-1-methylxanthine, IBMX; 300μM). Forskolin and IBMX also caused concentration-dependent increases in peak Ca2+ transients (fura-2) and cell shortening (edge-detector) measured simultaneously in field-stimulated myocytes (37°C). Similar effects were seen upon application of dibutyryl cAMP. In voltage-clamped myocytes, H-89 (2μM) decreased basal Ca2+ transients, contractions and underlying Ca2+ currents. H-89 also decreased diastolic Ca2+ and the gain of excitation–contraction coupling (Ca2+ release/Ca2+ current), especially at negative membrane potentials. This was independent of alterations in sarcoplasmic reticulum (SR) Ca2+ loading, as SR stores were unchanged by PKA inhibition. H-89 also decreased the frequency, amplitude and width of spontaneous Ca2+ sparks measured in quiescent myocytes (loaded with fluo-4), but increased time-to-peak. Thus, H-89 suppressed SR Ca2+ release by decreasing Ca2+ current and by reducing the gain of excitation–contraction coupling, in part by decreasing the size of individual Ca2+ release units. These data suggest that basal PKA activity enhances SR Ca2+ release in the absence of ß-adrenergic stimulation. This may depress contractile function in models such as aging, where the cAMP/PKA pathway is altered due to low basal cAMP levels.