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Habitat segregation among five species of darters was studied in the Thames River watershed of southwestern Ontario during May-September in 1977 and 1978. Direct underwater observations and quantitative net sampling demonstrated that each species occupied a wide range of habitats; however, some degree of segregation was found. Rainbow (Etheostoma caeruleum ) and Greenside (E. blennioides ) darters predominated in riffles and Johnny (E. nigrum ) and Blackside (Percina maculata ) darters were found mainly in pools or raceways. Fantail Darters (E. flabellare ) were common in both riffles and raceways.