Details

Autor(en) / Beteiligte

• White, Linda R.
• Anne B. L. Marthinsen
• Jakobsen, Karin
• Eik-Nes, Kristen B.

Titel

Response of Bovine Alveolar Macrophages in Vitro to Welding Fume Particles

Ist Teil von

• Environmental Health Perspectives, 1983-09, Vol.51, p.211-215

Ort / Verlag

United States: National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare

Erscheinungsjahr

1983

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• Alveolar macrophages (AM) from bovine lungs were exposed in culture to manual metal arc (MMA) welding fume particles, chromium (Cr), UICC chrysotile A or anatase for 17-20 hr. All the welding particle samples were more cytotoxic to AM than to anatase. Particles from the welding of mild steel with a rutile-coated electrode were less cytotoxic than those produced with a basic-coated electrode. Particles from the welding of stainless steel were slightly more cytotoxic, and much of this activity was probably due to CrVI. Selective release of N-acetyl-β-glucosaminidase (β-NAG) was only detected after exposure of AM to chrysotile. Supplementation of the incubation medium with 10% serum increased the viability of all exposed AM cultures, an effect not produced by serum albumin alone. Incubation of particle samples with dipalmitoyl phosphatidylcholine (DPPC) prior to addition to AM reduced the cytotoxicity of the "rutile" welding particles and of chrysotile.