Chemical communications (Cambridge, England), 2013-01, Vol.49 (50), p.5630-5632
2013
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe
- Ist Teil von
- Chemical communications (Cambridge, England), 2013-01, Vol.49 (50), p.5630-5632
- Ort / Verlag
- England
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1359-7345eISSN: 1364-548XDOI: 10.1039/c3cc41129jTitel-ID: cdi_proquest_miscellaneous_1356954762
- Format
- –
- Schlagworte
- Biological Assay, Biosensing Techniques, Coordination Complexes - chemistry, DNA Repair, G-Quadruplexes, Iridium - chemistry, Luminescence, Luminescent Measurements, Molecular Probe Techniques, Uracil-DNA Glycosidase - antagonists & inhibitors, Uracil-DNA Glycosidase - chemistry, Uracil-DNA Glycosidase - metabolism