Cerebral cortex (New York, N.Y. 1991), 2013-06, Vol.23 (6), p.1410-1423
2013
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Details
- Autor(en) / Beteiligte
- Titel
- WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration
- Ist Teil von
- Cerebral cortex (New York, N.Y. 1991), 2013-06, Vol.23 (6), p.1410-1423
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2013
- Quelle
- Oxford Journals 2020 Medicine
- Beschreibungen/Notizen
- Glia-guided migration (glia-guided locomotion) during radial migration is a characteristic yet unique mode of migration. In this process, the directionality of migration is predetermined by glial processes and not by growth cones. Prior to the initiation of glia-guided migration, migrating neurons transform from multipolar to bipolar, but the molecular mechanisms underlying this multipolar-bipolar transition and the commencement of glia-guided migration are not fully understood. Here, we demonstrate that the multipolar-bipolar transition is not solely a cell autonomous event; instead, the interaction of growth cones with glial processes plays an essential role. Time-lapse imaging with lattice assays reveals the importance of vigorously active growth cones in searching for appropriate glial scaffolds, completing the transition, and initiating glia-guided migration. These growth cone activities are regulated by Abl kinase and Cdk5 via WAVE2-Abi2 through the phosphorylation of tyrosine 150 and serine 137 of WAVE2. Neurons that do not display such growth cone activities are mispositioned in a more superficial location in the neocortex, suggesting the significance of growth cones for the final location of the neurons. This process occurs in spite of the "inside-out" principle in which later-born neurons are situated more superficially.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1047-3211eISSN: 1460-2199DOI: 10.1093/cercor/bhs123Titel-ID: cdi_proquest_miscellaneous_1356930786
- Format
- –
- Schlagworte
- Adaptor Proteins, Signal Transducing - metabolism, Age Factors, Animals, Cadherins - metabolism, Cell Movement - genetics, Cell Proliferation, Cells, Cultured, Cercopithecus aethiops, Cerebral Cortex - cytology, Dextran Sulfate - metabolism, Embryo, Mammalian, Female, Gene Expression Regulation, Developmental - genetics, Gene Transfer Techniques, Green Fluorescent Proteins - genetics, Green Fluorescent Proteins - metabolism, Growth Cones - physiology, Homeodomain Proteins - genetics, Homeodomain Proteins - metabolism, Immunoprecipitation, In Vitro Techniques, Male, Membrane Proteins - metabolism, Mice, Mice, Inbred C57BL, Mutation - genetics, Neuroglia - physiology, Neurons - cytology, Neurons - physiology, Pregnancy, RNA Interference - physiology, Transfection, Wiskott-Aldrich Syndrome Protein Family - genetics, Wiskott-Aldrich Syndrome Protein Family - metabolism