Details

Autor(en) / Beteiligte

• Xie, Wei
• Schultz, Matthew D.
• Lister, Ryan
• Hou, Zhonggang
• Rajagopal, Nisha
• Ray, Pradipta
• Whitaker, John W.
• Tian, Shulan
• Hawkins, R. David
• Leung, Danny
• Yang, Hongbo
• Wang, Tao
• Lee, Ah Young
• Swanson, Scott A.
• Zhang, Jiuchun
• Zhu, Yun
• Kim, Audrey
• Nery, Joseph R.
• Urich, Mark A.
• Kuan, Samantha
• Yen, Chia-an
• Klugman, Sarit
• Yu, Pengzhi
• Suknuntha, Kran
• Propson, Nicholas E.
• Chen, Huaming
• Edsall, Lee E.
• Wagner, Ulrich
• Li, Yan
• Ye, Zhen
• Kulkarni, Ashwinikumar
• Xuan, Zhenyu
• Chung, Wen-Yu
• Chi, Neil C.
• Antosiewicz-Bourget, Jessica E.
• Slukvin, Igor
• Stewart, Ron
• Zhang, Michael Q.
• Wang, Wei
• Thomson, James A.
• Ecker, Joseph R.
• Ren, Bing

Titel

Epigenomic Analysis of Multilineage Differentiation of Human Embryonic Stem Cells

Ist Teil von

• Cell, 2013-05, Vol.153 (5), p.1134-1148

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Epigenetic mechanisms have been proposed to play crucial roles in mammalian development, but their precise functions are only partially understood. To investigate epigenetic regulation of embryonic development, we differentiated human embryonic stem cells into mesendoderm, neural progenitor cells, trophoblast-like cells, and mesenchymal stem cells and systematically characterized DNA methylation, chromatin modifications, and the transcriptome in each lineage. We found that promoters that are active in early developmental stages tend to be CG rich and mainly engage H3K27me3 upon silencing in nonexpressing lineages. By contrast, promoters for genes expressed preferentially at later stages are often CG poor and primarily employ DNA methylation upon repression. Interestingly, the early developmental regulatory genes are often located in large genomic domains that are generally devoid of DNA methylation in most lineages, which we termed DNA methylation valleys (DMVs). Our results suggest that distinct epigenetic mechanisms regulate early and late stages of ES cell differentiation. [Display omitted] •Epigenome was mapped in depth for hESCs and four hESC-derived cell types•Lineage-restricted genes and regulatory sequences were identified in these cell types•Distinct mechanisms regulate lineage-restricted genes at early and late stages•Developmental genes tend to reside in large genomic domains devoid of DNA methylation DNA methylation, histone modifications, and the transcriptome have been mapped in human ES cells and four lineages. The results suggest that early and late stages of ES cell differentiation are regulated by distinct epigenetic mechanisms.