Details

Autor(en) / Beteiligte

• Shen, Wei
• Deng, Huimin
• Ren, Yuqian
• Gao, Zhiqiang

Titel

A real-time colorimetric assay for label-free detection of microRNAs down to sub-femtomolar levels

Ist Teil von

• Chemical communications (Cambridge, England), 2013-05, Vol.49 (43), p.4959-4961

Ort / Verlag

England

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• A real-time colorimetric assay utilizing gold nanoparticle networks and a duplex-specific nuclease (DSN) for label-free detection of microRNAs is proposed. Excellent sensitivity and selectivity were accomplished through the engagement of the DSN and a cumulative signal amplification process. A real-time microRNA assay is developed by leveraging on the amplification power of a duplex-specific nuclease and gold nanoparticle networks.