Details

Autor(en) / Beteiligte

• Wang, K W
• Chueh, L L
• Wang, M H
• Huang, Y T
• Fang, B H
• Chang, C Y
• Fang, M C
• Chou, J Y
• Hsieh, S C
• Wan, C H

Titel

Multiplex polymerase chain reaction assay for the detection of minute virus of mice and mouse parvovirus infections in laboratory mice

Ist Teil von

• Laboratory animals (London), 2013-04, Vol.47 (2), p.116-121

Ort / Verlag

London, England: SAGE Publications

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Mouse parvoviruses are among the most prevalent infectious pathogens in contemporary mouse colonies. To improve the efficiency of routine screening for mouse parvovirus infections, a multiplex polymerase chain reaction (PCR) assay targeting the VP gene was developed. The assay detected minute virus of mice (MVM), mouse parvovirus (MPV) and a mouse housekeeping gene (α-actin) and was able to specifically detect MVM and MPV at levels as low as 50 copies. Co-infection with the two viruses with up to 200-fold differences in viral concentrations can easily be detected. The multiplex PCR assay developed here could be a useful tool for monitoring mouse health and the viral contamination of biological materials.