Anticancer research, 2013-04, Vol.33 (4), p.1511-1517
2013
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Details
- Autor(en) / Beteiligte
- Titel
- miRNA expression profiles of oral squamous cell carcinomas
- Ist Teil von
- Anticancer research, 2013-04, Vol.33 (4), p.1511-1517
- Ort / Verlag
- Greece
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Oral squamous cell carcinoma (OSCC) development involves complex machinery of genomic and epigenetic regulations including microRNA (miRNA) expression changes. We aimed to analyze the expression patterns of a set of miRNAs in human TNM stage I and II OSCC samples in an autologous normal mucosa-controlled experimental design. Forty samples of OSCC and 40 matched normal tissues were evaluated for miR-21, miR-155, miR-191, miR-146a, miR-221 and miR-222 expression in a LightCycler 480(PCR) system. Our results showed significant overexpression of miR-21, miR-155, miR-191 and miR-221 in paired-sample t-test and the sensitivity/specificity of tests were over 90% in the case of miR-21 and miR-155 on the receiver operating characteristic curve (ROC) analysis. Our results underline the role of miR-21 in OSCC and support the possible causal role of miR-155 and miR-221 in oral carcinogenesis. The overexpression of miR-191 is a novel finding in squamous cell carcinoma.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1791-7530Titel-ID: cdi_proquest_miscellaneous_1324958066
- Format
- –
- Schlagworte
- Aged, Biomarkers, Tumor - genetics, Carcinoma, Squamous Cell - genetics, Carcinoma, Squamous Cell - pathology, Case-Control Studies, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Male, MicroRNAs - genetics, Middle Aged, Mouth Mucosa - metabolism, Mouth Neoplasms - genetics, Mouth Neoplasms - pathology, Neoplasm Staging, Oligonucleotide Array Sequence Analysis, Prognosis, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - genetics