Details

Autor(en) / Beteiligte

• Hobson, Neil
• Deyholos, Michael K

Titel

LuFLA1 sub(PRO) and LuBGAL1 sub(PRO) promote gene expression in the phloem fibres of flax (Linum usitatissimum)

Ist Teil von

• Plant cell reports, 2013-04, Vol.32 (4), p.517-528

Erscheinungsjahr

2013

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Key message : Cell type-specific promoters were identified that drive gene expression in an industrially important product. Abstract: To identify flax (Linum usitatissimum) gene promoters, we analyzed the genomic regions upstream of a fasciclin-like arabinogalactan protein (LuFLA1) and a beta-galactosidase (LuBGAL1). Both of these genes encode transcripts that have been found to be highly enriched in tissues bearing phloem fibres. Using a beta-glucuronidase (GUS) reporter construct, we found that a 908-bp genomic sequence upstream of LuFLA1 (LuFLA1 sub(PRO)) directed GUS expression with high specificity to phloem fibres undergoing secondary cell wall development. The DNA sequence upstream of LuBGAL1 (LuBGAL1 sub(PRO)) likewise produced GUS staining in phloem fibres with developing secondary walls, as well as in tissues of developing flowers and seed bolls. These data provide further evidence of a specific role for LuFLA1 in phloem fibre development, and demonstrate the utility of LuFLA1 sub(PRO) and LuBGAL1 sub(PRO) as tools for biotechnology and further investigations of phloem fibre development.