UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 12 von 37
Datensatz exportieren als...
BibTeX
Tumor necrosis factor α suppresses the mesenchymal stem cell osteogenesis promoter miR‐21 in estrogen deficiency–induced osteoporosis
Journal of bone and mineral research, 2013-03, Vol.28 (3), p.559-573
Yang, Nan
Wang, Guang
Hu, Chenghu
Shi, Yuanyuan
Liao, Li
Shi, Songtao
Cai, Yan
Cheng, Shuli
Wang, Xi
Liu, Yali
Tang, Liang
Ding, Yin
Jin, Yan
2013
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Yang, Nan
Wang, Guang
Hu, Chenghu
Shi, Yuanyuan
Liao, Li
Shi, Songtao
Cai, Yan
Cheng, Shuli
Wang, Xi
Liu, Yali
Tang, Liang
Ding, Yin
Jin, Yan
Titel
Tumor necrosis factor α suppresses the mesenchymal stem cell osteogenesis promoter miR‐21 in estrogen deficiency–induced osteoporosis
Ist Teil von
Journal of bone and mineral research, 2013-03, Vol.28 (3), p.559-573
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2013
Quelle
MEDLINE
Beschreibungen/Notizen
Inflammatory cytokines, especially tumor necrosis factor α (TNF‐α), have been shown to inhibit osteogenic differentiation of mesenchymal stem cells (MSCs) and bone formation in estrogen deficiency–induced osteoporosis, but the mechanism responsible remains poorly understood. MicroRNAs (miRNAs) have been shown to regulate MSC differentiation. Here, we identified a novel mechanism whereby TNF‐α, suppressing the functional axis of a key miRNA (miR‐21) contributes to estrogen deficiency–induced osteoporosis. In this study, we screened differentially expressed miRNAs in MSCs derived from estrogen deficiency‐induced osteoporosis and found miR‐21 was significantly downregulated. miR‐21 was suppressed by TNF‐α during the osteogenesis of MSCs. Furthermore, miR‐21 was confirmed to promote the osteoblast differentiation of MSCs by repressing Spry1, which can negatively regulate the osteogenic differentiation of MSCs. Upregulating miR‐21 partially rescued TNF‐α–impaired osteogenesis of MSCs. Blocking TNF‐α ameliorated the inflammatory environment and significantly enhanced bone formation with increased miR‐21 expression and suppressed Spry1 expression in ovariectomized (OVX) mice. Our results revealed a novel function for miR‐21 and suggested that suppressed miR‐21 may contribute to impaired bone formation by elevated TNF‐α in estrogen deficiency–induced osteoporosis. This study may indicate a molecular basis for novel therapeutic strategies against osteoporosis and other inflammatory bone diseases. © 2013 American Society for Bone and Mineral Research.
Sprache
Englisch
Identifikatoren
ISSN: 0884-0431
eISSN: 1523-4681
DOI: 10.1002/jbmr.1798
Titel-ID: cdi_proquest_miscellaneous_1323815274
Format
–
Schlagworte
Animals
,
Bone diseases
,
Cell Differentiation
,
Cytokines
,
Down-Regulation
,
Enzyme-Linked Immunosorbent Assay
,
Estrogens
,
Female
,
Humans
,
Inflammation
,
Inflammatory diseases
,
MESENCHYMAL STEM CELL
,
Mesenchymal Stromal Cells - cytology
,
Mesenchymal Stromal Cells - metabolism
,
Mesenchyme
,
Mice
,
MicroRNAs
,
miRNA
,
miR‐21
,
Osteoblastogenesis
,
Osteoblasts - cytology
,
Osteoblasts - metabolism
,
OSTEOGENESIS
,
Osteogenesis - genetics
,
OSTEOPOROSIS
,
Ovariectomy
,
Promoters
,
Stem cells
,
TNF‐α
,
Tumor necrosis factor- alpha
,
Tumor Necrosis Factor-alpha - physiology
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX