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Nature biotechnology, 2013-03, Vol.31 (3), p.230-232

2013

Autor(en) / Beteiligte

Titel

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Ist Teil von

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.

Sprache: Englisch
Identifikatoren: ISSN: 1087-0156
eISSN: 1546-1696
DOI: 10.1038/nbt.2507
Titel-ID: cdi_proquest_miscellaneous_1323804891

Format: –
Schlagworte: Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Base Sequence, Biotechnology, CRISPR-Cas Systems, DNA Cleavage, DNA, Recombinant - genetics, DNA, Recombinant - metabolism, Endonucleases - genetics, Endonucleases - metabolism, Genes, Reporter, Genetic aspects, Genetic engineering, Genetic Engineering - methods, Genome, Human, Histocompatibility Antigens - genetics, Histocompatibility Antigens - metabolism, Humans, Immunology, Luminescent Proteins - genetics, Luminescent Proteins - metabolism, Methods, Molecular Sequence Data, Mutagenesis, Site-Directed, Physiological aspects, Receptors, CCR5 - genetics, Receptors, CCR5 - metabolism, Restriction enzymes, DNA, Restriction-modification system, Ribonucleic acid, RNA, RNA, Guide - genetics, RNA, Guide - metabolism, Streptococcus pyogenes, Streptococcus pyogenes - enzymology, Streptococcus pyogenes - genetics, Zinc Fingers

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