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Cellular Mechanotransduction Relies on Tension-Induced and Chaperone-Assisted Autophagy
Ist Teil von
Current biology, 2013-03, Vol.23 (5), p.430-435
Ort / Verlag
England: Elsevier Inc
Erscheinungsjahr
2013
Quelle
EZB-FREE-00999 freely available EZB journals
Beschreibungen/Notizen
Mechanical tension is an ever-present physiological stimulus essential for the development and homeostasis of locomotory, cardiovascular, respiratory, and urogenital systems [1, 2]. Tension sensing contributes to stem cell differentiation, immune cell recruitment, and tumorigenesis [3, 4]. Yet, how mechanical signals are transduced inside cells remains poorly understood. Here, we identify chaperone-assisted selective autophagy (CASA) as a tension-induced autophagy pathway essential for mechanotransduction in muscle and immune cells. The CASA complex, comprised of the molecular chaperones Hsc70 and HspB8 and the cochaperone BAG3, senses the mechanical unfolding of the actin-crosslinking protein filamin. Together with the chaperone-associated ubiquitin ligase CHIP, the complex initiates the ubiquitin-dependent autophagic sorting of damaged filamin to lysosomes for degradation. Autophagosome formation during CASA depends on an interaction of BAG3 with synaptopodin-2 (SYNPO2). This interaction is mediated by the BAG3 WW domain and facilitates cooperation with an autophagosome membrane fusion complex. BAG3 also utilizes its WW domain to engage in YAP/TAZ signaling. Via this pathway, BAG3 stimulates filamin transcription to maintain actin anchoring and crosslinking under mechanical tension. By integrating tension sensing, autophagosome formation, and transcription regulation during mechanotransduction, the CASA machinery ensures tissue homeostasis and regulates fundamental cellular processes such as adhesion, migration, and proliferation.
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► Chaperone-assisted selective autophagy is a tension-induced degradation pathway ► BAG3 uses its WW domain to bind PPxY proteins, including SYNPO2 and YAP/TAZ inhibitors ► SYNPO2 couples client ubiquitylation to autophagosome formation ► BAG3 stimulates YAP/TAZ-mediated transcription under tension