Details

Autor(en) / Beteiligte

• Calvignac-Spencer, Sébastien
• Merkel, Kevin
• Kutzner, Nadine
• Kühl, Hjalmar
• Boesch, Christophe
• Kappeler, Peter M.
• Metzger, Sonja
• Schubert, Grit
• Leendertz, Fabian H.

Titel

Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity

Ist Teil von

• Molecular ecology, 2013-02, Vol.22 (4), p.915-924

Ort / Verlag

Oxford: Blackwell Publishing Ltd

Erscheinungsjahr

2013

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Large‐scale monitoring schemes are essential in assessing global mammalian biodiversity, and in this framework, leeches have recently been promoted as an indirect source of DNA from terrestrial mammal species. Carrion feeding flies are ubiquitous and can be expected to feed on many vertebrate carcasses. Hence, we tested whether fly‐derived DNA analysis may also serve as a novel tool for mammalian diversity surveys. We screened DNA extracted from 201 carrion flies collected in tropical habitats of Côte d'Ivoire and Madagascar for mammal DNA using multiple PCR systems and retrieved DNA sequences from a diverse set of species (22 in Côte d'Ivoire, four in Madagascar) exploiting distinct forest strata and displaying a broad range of body sizes. Deep sequencing of amplicons generated from pools of flies performed equally well as individual sequencing approaches. We conclude that the analysis of fly‐derived DNA can be implemented in a very rapid and cost‐effective manner and will give a relatively unbiased picture of local mammal diversity. Carrion flies therefore represent an extraordinary and thus far unexploited resource of mammal DNA, which will probably prove useful for future inventories of wild mammal communities. See also the Perspective by Bohmann et al.