Details

Autor(en) / Beteiligte

• Shao, Yuan-jun
• Hu, Xian-qiong
• Peng, Guang-da
• Wang, Rui-xian
• Gao, Rui-na
• Lin, Chao
• Shen, Wei-de
• Li, Rui
• Li, Bing

Titel

Structure and evolution of the mitochondrial genome of Exorista sorbillans: the Tachinidae (Diptera: Calyptratae) perspective

Ist Teil von

• Molecular biology reports, 2012-12, Vol.39 (12), p.11023-11030

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• The first complete mitochondrial genome (mitogenome) of Tachinidae Exorista sorbillans (Diptera) is sequenced by PCR-based approach. The circular mitogenome is 14,960 bp long and has the representative mitochondrial gene (mt gene) organization and order of Diptera. All protein-coding sequences are initiated with ATN codon; however, the only exception is Cox I gene, which has a 4-bp ATCG putative start codon. Ten of the thirteen protein-coding genes have a complete termination codon (TAA), but the rest are seated on the H strand with incomplete codons. The mitogenome of E . sorbillans is biased toward A+T content at 78.4 %, and the strand-specific bias is in reflection of the third codon positions of mt genes, and their T/C ratios as strand indictor are higher on the H strand more than those on the L strand pointing at any strain of seven Diptera flies. The length of the A+T-rich region of E. sorbillans is 106 bp, including a tandem triple copies of a13-bp fragment. Compared to Haematobia irritans , E. sorbillans holds distant relationship with Drosophila . Phylogenetic topologies based on the amino acid sequences, supporting that E. sorbillans (Tachinidae) is clustered with strains of Calliphoridae and Oestridae, and superfamily Oestroidea are polyphyletic groups with Muscidae in a clade.