Current biology, 2013-02, Vol.23 (4), p.290-297
2013
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- Autor(en) / Beteiligte
- Titel
- Rho GTPase Signaling Activates Microtubule Severing to Promote Microtubule Ordering in Arabidopsis
- Ist Teil von
- Current biology, 2013-02, Vol.23 (4), p.290-297
- Ort / Verlag
- England: Elsevier Inc
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Ordered cortical microtubule (MT) arrays play a critical role in the spatial control of cell division and expansion and are essential for plant growth, morphogenesis, and development. Various developmental, hormonal, and mechanical signals and a large number of MT-associated proteins are known to impact cortical MT organization, but the underlying mechanisms remain poorly understood. Our previous studies show that auxin signaling, which is mediated by the ROP6 Rho GTPase and its effector RIC1, promotes the ordering of cortical MTs in pavement cells, but it is unknown how RIC1 controls the organization of cortical MTs into well-ordered arrays. Our genetic screens identified the conserved MT-severing protein katanin (KTN1) as a downstream component of the ROP6-RIC1 signaling pathway leading to well-ordered arrangement of cortical MTs. KTN1 and RIC1 proteins displayed overlapping localization. In vivo and in vitro studies showed that RIC1 physically interacts with and promotes the MT-severing activity of KTN1. Live-cell imaging reveals a role for RIC1 in promoting detachment of branched MTs that is known to rely on KTN1. We have demonstrated that a Rho GTPase signaling pathway regulates katanin-mediated MT severing in plant cells and uncovered an explicit regulatory mechanism underpinning the alignment and ordering of cortical MTs in plants. Our findings provide new insights into regulatory mechanisms underlying growth stimuli such as auxin promote the organization of cortical MTs into parallel arrays in plants. ► The KTN1 katanin genetically acts in the ROP6-RIC1 pathway ► KTN1 is required for ROP6- and RIC1-dependent microtubule ordering ► RIC1 interacts with KTN1 and promotes its microtubule severing activity ► RIC1 promotes the detachment of branched MTs in vivo
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-9822eISSN: 1879-0445DOI: 10.1016/j.cub.2013.01.022Titel-ID: cdi_proquest_miscellaneous_1312176298
- Format
- –
- Schlagworte
- Adenosine Triphosphatases - genetics, Adenosine Triphosphatases - metabolism, Arabidopsis, Arabidopsis - genetics, Arabidopsis - growth & development, Arabidopsis - metabolism, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, auxins, cell division, cells, guanosinetriphosphatase, image analysis, in vitro studies, Indoleacetic Acids - metabolism, Katanin, Microtubule-Associated Proteins - genetics, Microtubule-Associated Proteins - metabolism, Microtubules - genetics, Microtubules - metabolism, Molecular Sequence Data, Monomeric GTP-Binding Proteins - genetics, Monomeric GTP-Binding Proteins - metabolism, morphogenesis, Mutation, plant growth, proteins, rho GTP-Binding Proteins - metabolism, Signal Transduction