Cellular immunology, 2012-11, Vol.280 (1), p.1-9
2012
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- Autor(en) / Beteiligte
- Titel
- VDUP1 exacerbates bacteremic shock in mice infected with Pseudomonas aeruginosa
- Ist Teil von
- Cellular immunology, 2012-11, Vol.280 (1), p.1-9
- Ort / Verlag
- Netherlands: Elsevier Inc
- Erscheinungsjahr
- 2012
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- ► The roles of VDUP1 in Pseudomonas aeruginosa (P. aeruginosa) infection were examined. ► VDUP1 aggravated P. aeruginosa infection. ► In addition, VDUP1 suppressed bacterial clearance and ROS generation. Vitamin-D3 upregulated protein-1 (VDUP1) is a stress response protein. Pseudomonas aeruginosa (P. aeruginosa) infection is a leading cause of death. Mice infected with live P. aeruginosa exhibit significantly decreased VDUP1 expression. However, the function of VDUP1 during P. aeruginosa-induced mouse bacteremic shock is unknown. To address the function of VDUP1 in P. aeruginosa-infected mice, we constructed a bacteremic shock model wherein both wild-type and VDUP1-deficient mice were infected intra-peritoneally with live P. aeruginosa. We found that VDUP1-deficient mice were more resistant to P. aeruginosa-induced bacteremic shock than wild-type mice, as shown by the increased survival, accelerated bacterial clearance and suppression of cytokine overproduction of the VDUP1-deficient mice. VDUP1 promoted the recruitment of neutrophils into the peritoneal cavities of infected mice. VDUP1 impeded the phagocytosis of non-opsonized P. aeruginosa via phosphatidylinositide 3-kinase (PI3K) pathway in macrophages. P. aeruginosa infection induced the generation of reactive oxygen species (ROS), and the increased production of ROS by the peritoneal cells of VDUP1-deficient mice was advantageous in clearing the bacteria. Overall, VDUP1 aggravates bacteremic shock; thus, VDUP1 can be considered a target molecule for the inhibition of P. aeruginosa-induced bacteremic shock.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0008-8749eISSN: 1090-2163DOI: 10.1016/j.cellimm.2012.11.003Titel-ID: cdi_proquest_miscellaneous_1291605479
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, Animal models, Animals, Apoptosis - physiology, Bacteremic shock, Carrier Proteins - genetics, Carrier Proteins - physiology, Chemotaxis, Leukocyte - physiology, Colony Count, Microbial, Cytokines, Cytokines - biosynthesis, Infection, Leukocytes (neutrophilic), Macrophages, Mice, Mice, Inbred C57BL, Mice, Knockout, Neutrophils - pathology, P. aeruginosa, Peritoneal Cavity - microbiology, Peritoneal Cavity - pathology, Peritoneum, Peritonitis - microbiology, Peritonitis - physiopathology, Phagocytosis, Phagocytosis - physiology, phosphatidylinositides, Phosphatidylinositol 3-Kinases - physiology, Pseudomonas aeruginosa, Pseudomonas aeruginosa - growth & development, Pseudomonas aeruginosa - isolation & purification, Pseudomonas Infections - physiopathology, Reactive oxygen species, Reactive Oxygen Species - metabolism, ROS, Shock, Shock, Septic - physiopathology, Signal Transduction - physiology, Specific Pathogen-Free Organisms, Spleen - microbiology, Stress, Thioredoxins - genetics, Thioredoxins - physiology, VDUP1