Cancer research (Chicago, Ill.), 2013-02, Vol.73 (4), p.1276-1286
2013
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Details
- Autor(en) / Beteiligte
- Titel
- Nitroreductase, a Near-Infrared Reporter Platform for In Vivo Time-Domain Optical Imaging of Metastatic Cancer
- Ist Teil von
- Cancer research (Chicago, Ill.), 2013-02, Vol.73 (4), p.1276-1286
- Ort / Verlag
- Philadelphia, PA: American Association for Cancer Research
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The ability to visualize reporter gene expression in vivo has revolutionized all facets of biologic investigation and none more so than imaging applications in oncology. Near-infrared reporter gene imaging may facilitate more accurate evaluation of chemotherapeutic response in preclinical models of orthotopic and metastatic cancers. We report the development of a cell permeable, quenched squarine probe (CytoCy5S), which is reduced by Escherichia coli nitroreductase (NTR), resulting in a near-infrared fluorescent product. Time-domain molecular imaging of NTR/CytoCy5S reporter platform permitted noninvasive monitoring of disease progression in orthotopic xenografts of disseminated leukemia, lung, and metastatic breast cancer. This methodology facilitated therapeutic evaluation of NTR gene-directed enzymatic prodrug therapy with conventional metronidazole antibiotics. These studies show NTR/CytoCy5S as a near-infrared gene reporter system with broad preclinical and prospective clinical applications within imaging, and gene therapy, of cancer.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0008-5472eISSN: 1538-7445DOI: 10.1158/0008-5472.can-12-2649Titel-ID: cdi_proquest_miscellaneous_1288994993
- Format
- –
- Schlagworte
- Animals, Anti-Infective Agents - pharmacology, Antineoplastic agents, Biological and medical sciences, Carbocyanines - chemistry, Carbocyanines - metabolism, Cell Line, Tumor, Cell Survival - drug effects, Escherichia coli Proteins - chemistry, Escherichia coli Proteins - genetics, Escherichia coli Proteins - metabolism, Green Fluorescent Proteins - genetics, Green Fluorescent Proteins - metabolism, HEK293 Cells, Humans, Luciferases - genetics, Luciferases - metabolism, Magnetic Resonance Imaging - methods, Medical sciences, Metronidazole - metabolism, Metronidazole - pharmacology, Mice, Mice, Inbred NOD, Mice, SCID, Microscopy, Fluorescence - methods, Neoplasm Metastasis, Neoplasms - drug therapy, Neoplasms - genetics, Neoplasms - metabolism, Nitroimidazoles - metabolism, Nitroimidazoles - pharmacology, Nitroreductases - chemistry, Nitroreductases - genetics, Nitroreductases - metabolism, Pharmacology. Drug treatments, Reproducibility of Results, Transfection, Tumors, Xenograft Model Antitumor Assays