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The effects of a 1.8 GHz continuous electromagnetic fields on mucociliary transport of human nasal mucosa
Ist Teil von
The Laryngoscope, 2013-02, Vol.123 (2), p.315-320
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2013
Link zum Volltext
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
Objectives/Hypothesis:
The aim of this study was to investigate the effects of a 1.8 GHz continuous electromagnetic fields (EMF) on human nasal mucociliary transport, and to determine the pathophysiology of ciliary beat frequency (CBF) during an EMF‐induced change.
Methods:
Human nasal mucosa cells were exposed to a 1.8 GHz EMF (SAR=1.0 W/kg), and CBF was analyzed using an optical flow technique with the peak detection method.
Results:
The 1.8 GHz‐exposed group showed a decreased CBF when compared to the control group. In the cytotoxicity assay, difference in survival rates was not found between the two groups. In the EMF‐exposed group, protein kinase C (PKC) activity was increased during a PKC activity assay. The broad PKC inhibitor, Calphostin C abolished the EMF‐induced decrease of CBF. The EMF‐induced decrease of CBF was abolished by GF 109203X, a novel PKC (nPKC) isoform inhibitor, whereas the decrease was not attenuated by Gö‐6976, a specific inhibitor of conventional PKC (cPKC) isoform.
Conclusions:
EMF may inhibit CBF via an nPKC‐dependent mechanism. Therefore, we have confirmed that EMF could decrease CBF by increasing PKC activity. Laryngoscope, 2012