Biochemistry (Moscow), 2012-11, Vol.77 (11), p.1315-1325
2012
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Details
- Autor(en) / Beteiligte
- Titel
- Molecular cloning, isolation, and properties of chaperone Skp from Yersinia pseudotuberculosis
- Ist Teil von
- Biochemistry (Moscow), 2012-11, Vol.77 (11), p.1315-1325
- Ort / Verlag
- Dordrecht: SP MAIK Nauka/Interperiodica
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The skp gene of Yersinia pseudotuberculosis was expressed without its signal sequence in Escherichia coli BL21(DE3) cells. The recombinant protein Skp accumulated in soluble form in the cytoplasm of the producer strain. The protein was isolated and characterized: the molecular weight, isoelectric point, N-terminal amino acid sequence (20 amino acid residues), and the content of the secondary structure elements were determined. Using cross-linking stabilization and high-mass MALDI-TOF mass spectrometric analysis, it was found that rSkp forms a stable homotrimer in solution and interacts with human IgG. Three-dimensional models of the Skp trimer and its complexes with Fc- and Fab-fragments of human IgG1 were constructed by computer modeling.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-2979eISSN: 1608-3040DOI: 10.1134/S0006297912110119Titel-ID: cdi_proquest_miscellaneous_1285088310
- Format
- –
- Schlagworte
- Amino Acid Sequence, Amino acids, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Biochemistry, Biomedical and Life Sciences, Biomedicine, Bioorganic Chemistry, Circular Dichroism, Cloning, Cloning, Molecular, E coli, Escherichia coli, Escherichia coli - metabolism, Gene expression, Humans, Immunoglobulin G, Immunoglobulin G - metabolism, Immunoglobulins, Life Sciences, Mass spectrometry, Microbiology, Molecular biology, Molecular Chaperones - chemistry, Molecular Chaperones - genetics, Molecular Chaperones - metabolism, Molecular Sequence Data, Protein Structure, Quaternary, Proteins, Recombinant proteins, Recombinant Proteins - biosynthesis, Recombinant Proteins - chemistry, Recombinant Proteins - isolation & purification, Sequence Alignment, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Yersinia pseudotuberculosis, Yersinia pseudotuberculosis - metabolism