Details

Autor(en) / Beteiligte

• Urrutia, Paulina
• Rodriguez-Colinas, Bárbara
• Fernandez-Arrojo, Lucía
• Ballesteros, Antonio O
• Wilson, Lorena
• Illanes, Andrés
• Plou, Francisco J

Titel

Detailed Analysis of Galactooligosaccharides Synthesis with β‑Galactosidase from Aspergillus oryzae

Ist Teil von

• Journal of agricultural and food chemistry, 2013-02, Vol.61 (5), p.1081-1087

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The synthesis of galactooligosaccharides (GOS) catalyzed by β-galactosidase from Aspergillus oryzae (Enzeco) was studied. Using 400 g/L of lactose and 15 U/mL, maximum GOS yield, measured by HPAEC-PAD, was 26.8% w/w of total carbohydrates, obtained at approximately 70% lactose conversion. No less than 17 carbohydrates were identified; the major transgalactosylation product was 6′-O-β-galactosyl-lactose, representing nearly one-third (in weight) of total GOS. In contrast with previous reports, the presence of at least five disaccharides was detected, which accounted for 40% of the total GOS at the point of maximum GOS concentration (allolactose and 6-galactobiose were the major products). A. oryzae β-galactosidase showed a preference to form β(1→6) bonds, followed by β(1→3) and β(1→4) linkages. Results were compared with those obtained with β-galactosidases from Kluyveromyces lactis and Bacillus circulans. The highest GOS yield and specific productivity were achieved with B. circulans β-galactosidase. The specificity of the linkages formed and distribution of di-, tri-, and higher GOS varied significantly among the three β-galactosidases.