Details

Autor(en) / Beteiligte

• Fraczek, M
• Rostkowska-Nadolska, B
• Kusmierz, D
• Zielinska, A
• Rok, J
• Sliupkas-Dyrda, E
• Grzanka, A
• Krecicki, T
• Latocha, M

Titel

Vitamin D analogs decrease in vitro secretion of RANTES and enhance the effect of budesonide

Ist Teil von

• Advances in medical sciences, 2012-12, Vol.57 (2), p.290-295

Ort / Verlag

Netherlands: Elsevier Urban & Partner Sp. z o.o

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Eosinophils appear to be central inflammatory cells in the pathogenesis of rhinosinusitis with nasal polyps (NP). One of the most predominantly recognized eosinophil chemoattractants is RANTES. The aim of this study was to assess the influence of vitamin D (VD) derivates on RANTES expression in the culture of nasal polyp fibroblasts. NP fibroblast cell cultures derived from 16 patients with NP were first stimulated with bacterial LPS and than incubated in increasing concentrations (from 10−7M to 10−4M) of calcitriol, tacalcitol or budesonide and in combination with one of VD derivate with budesonide in 1:1, 1:3 and 3:1 ratios. Quantitative analysis of RANTES level was conducted in culture supernatants using an ELISA method. The highest calcitriol concentration (10−4M) as well as tacalcitol at 10−5M and 10−4M reduced RANTES production significantly compared to the control (201.1pg/ml, 338.7pg/ml, 211.3pg/ml v 571.78pg/ml; p<0.05). Budesonide and calcitriol administered in 1:3 ratio and budesonide and tacalcitol in 1:1 and 1:3 reduced RANTES concentration significantly better than each of the drug used in monotherapy (p<0.05). Budesonide and tacalcitol in 1:1 and 1:3 ratios suppressed RANTES production to the lowest level (171.8±97.6pg/ml and 178.7±105.22pg/ml, respectively). Active VD compounds via downregulation of RANTES production exert a potential role as a complementary element in the therapy of chronic rhinosinusitis with NP. Compounds consisting of budesonide and VD derivate have an advantage over both drugs used in monotherapy.