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BjHO-1 is involved in the detoxification of heavy metal in India mustard (Brassica juncea)
Ist Teil von
Biometals, 2012-12, Vol.25 (6), p.1269-1279
Ort / Verlag
Dordrecht: Springer Netherlands
Erscheinungsjahr
2012
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Heme oxygenase
-
1
(
HO
-
1
) is a stress-responsive gene coding for an enzyme catalyzing the catabolism of heme to yield biliverdin IXα, carbon monoxide (CO) and iron. However, its biological role in regulating metal homeostasis, particularly the tolerance to toxic heavy metals is poorly understood. In this study, a novel gene encoding a
Brassica juncea
heme oxygenase-1 (designated as BjHO-1) was cloned and functionally identified. Spatial expression of
BjHO
-
1
showed that it was differentially expressed in cotyledon, hypocotyl, leaf and root.
BjHO
-
1
was found to be induced significantly by heavy metal Hg. To understand whether
BjHO
-
1
is able to regulate plant tolerance to Hg, we constructed transgenic
B. juncea
plants overexpressing
HO
-
1
, and showed that
35S::BjHO1
plants confer the plant resistance to Hg toxicity by improving plant dry mass, reducing Hg accumulation, and attenuating Hg-induced oxidative stress. We further cloned a 1,099 bp promoter sequence upstream of
BjHO
-
1
using genome walking approach. Multiple stress-responsive elements were detected in the
BjHO
-
1
promoter regions. The promoter can be activated by Zn, Cd, Hg and Pb exposure. Our results indicate that up-regulation of
BjHO
-
1
is beneficial for limiting the uptake or accumulation of heavy metals into plants. This work also provides a new example for molecular breeding designed for plants that do not accumulate or minimizing accumulation of toxic trace metals growing on heavy metal-contaminated soils.