Details

Autor(en) / Beteiligte

• Chen, Zheng
• Bertin, Riccardo
• Froldi, Guglielmina

Titel

EC50 estimation of antioxidant activity in DPPH assay using several statistical programs

Ist Teil von

• Food chemistry, 2013-05, Vol.138 (1), p.414-420

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• ► DPPH assay allows the evaluation of antioxidant capacity through the determination of EC50. ► Different antioxidants were studied with DPPH assay ► The EC50 estimation was performed by GraphPad Prism, BLeSq, OriginPro, SigmaPlot, BioDataFit, and IBM SPSS programs. ► The statistical estimation gave similar EC50, but the five-parameter GraphPad Prism showed the best performance. DPPH assay is a reliable method to determine the antioxidant capacity of biological substrates. The DPPH radical scavenging activity is generally quantified in terms of inhibition percentage of the pre-formed free radical by antioxidants, and the EC50 (concentration required to obtain a 50% antioxidant effect) is a typically employed parameter to express the antioxidant capacity and to compare the activity of different compounds. In this study, the EC50 estimation was performed using a comparative approach based on various regression models implemented in six specialized computer programs: GraphPad Prism® version 5.01, BLeSq, OriginPro® 8.5.1, SigmaPlot® 12, BioDataFit® 1.02, and IBM SPSS Statistics® Desktop 19.0. For this project, quercetin, catechin, ascorbic acid, caffeic acid, chlorogenic acid and acetylcysteine were screened as antioxidant standards with DPPH assay to define the EC50 parameters. All the statistical programs gave similar EC50 values, but GraphPad Prism® five-parameter analysis pointed out a best performance, also showing a minor variance in relation to the actual EC50.