Details

Autor(en) / Beteiligte

• Wu, Hui
• Fu, YongMing
• Xiao, Jun
• Zhou, Man
• Zhou, Wei
• Feng, Hao

Titel

The unsulfated extracellular N-terminus of vGPCR reduces the tumorigenicity of hGRO-α in nude mice

Ist Teil von

• Science China. Life sciences, 2013, Vol.56 (1), p.26-31

Ort / Verlag

Beijing: Science China Press

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• The Kaposi＇s Sarcoma-associated Herpesvirus （KSHV）-encoded G-protein coupled receptor （vGPCR） is an oncoprotein that is implicated in KSHV-associated malignancies. We previously revealed vGPCR incorporates sulfate groups within its extracellular N-terminal tyrosine residues （Y26 and Y28） and that this tyrosine sulfation is crucial for its tumorigenicity in nude mice. hGRO-α binds vGPCR in a sulfotyrosine-dependent manner and promotes its tumorigenicity through autocrine signaling. Interestingly, an unsulfated vGPCR mutant （yydd-vGPCR） attenuated the tumor growth triggered by hGRO-α. In this study, the extracellular N-terminus of vGPCR （wt-vGN） and an unsulfated vGPCR mutant （yydd-vGN） were individually secreted, ex- pressed and purified. A radioactive labeling assay demonstrated that wt-vGN but not yydd-vGN incorporated [35S]-sulfate. In nude mice, NIH3T3 cells expressing yydd-vGN but not wt-vGN could significantly inhibit the tumor growth triggered by hGRO-α. All our data support the conclusion that the unsulfated extracellular N-terminus of vGPCR reduces the tumorigenicity of hGRO-α.