Details

Autor(en) / Beteiligte

• Ludwig, C
• Wachter, B
• Silinski-Mehr, S
• Ganswindt, A
• Bertschinger, H
• Hofer, H
• Dehnhard, M

Titel

Characterisation and validation of an enzyme-immunoassay for the non-invasive assessment of faecal glucocorticoid metabolites in cheetahs ( Acinonyx jubatus )

Ist Teil von

• General and comparative endocrinology, 2013-01, Vol.180 (1), p.15-23

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Graphical abstract We developed an enzyme immunoassay (EIA) based on an antibody directed against corticosterone-3-CMO which is a reliable tool to assess faecal lucocorticoid metabolites (fGCM) in cheetahs. Faecal GCM concentrations measured with the corticosterone-3-CMO assay before and after ACTH administration in three female and four male cheetahs. Concentrations are expressed as % (mean ± SEM) from the pre-challenge baseline. Highlights ► We tested four enzyme immunoassays (EIA) in two captive cheetahs to monitor adrenocortical activity. ► The assay comparison was carried out based on two ACTH challenges. ► The corticosterone-3-CMO EIA can be used to reliably assess faecal glucocorticoid (fGM) concentrations in cheetahs. ► A radiometabolism study used to identify fGM revealed a polar peak of radiolabelled cortisol metabolites. ► We suggest that these metabolites were conjugated, because solvolysis released free, unpolar metabolites.