Details

Autor(en) / Beteiligte

• Yu, Yanyan
• Yang, Yan
• Gu, Hui
• Zhou, Tianshu
• Shi, Guoyue

Titel

Size-tunable Pt nanoparticles assembled on functionalized ordered mesoporous carbon for the simultaneous and on-line detection of glucose and L-lactate in brain microdialysate

Ist Teil von

• Biosensors & bioelectronics, 2013-03, Vol.41, p.511-518

Ort / Verlag

Kidlington: Elsevier B.V

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• This study presents a facile electrochemical method for simultaneous and selective on-line detection of glucose and L-lactate in the striatum of anesthetic rats through the integration of selective electrochemical detection with in vivo microdialysis system. A positively-charged polyelectrolyte, (diallyldimethylammonium chloride) (PDDA), was attached onto carbon mesoporous material (CMM) through non-covalent interaction, which provided an ideal environment for the assembling and dispersion of nanoparticle electrocatalysts. Platinum nanoparticles with wide loadings from 5 to 50wt% were successfully self-assembled on PDDA-functionalized CMM via electrostatic interaction. TEM results showed that with the increase in the Pt loadings, both the size and interconnectivity between particles increased, with particle sizes ranging from 3.2±0.4 to 6.8±1.4nm. Moreover, the electrocatalytic activities of the as-prepared six Pt/PDDA-CMM hybrid nanocomposites were also observed to show an inverted-V-shaped profile as a function of loading amount of Pt NPs. Integrated with glucose oxidase (GOx), L-lactate oxidase (LOD) and the in vivo microdialysis system, the constructed dual oxidase/Pt/PDDA-CMM/Nafion biosensors were successfully applied for the simultaneous and on-line detection of glucose and L-lactate. After post-calibration, the basal level of glucose and L-lactate in the striatum of anesthetic rats was calculated to be 0.27±0.03 and 0.71±0.05mM (mean ±s.d., n=3), respectively. What is more important, the dual oxidase biosensors almost suffered from little cross-talk, which is characteristic of an excellent sensor with high performance. This property, along with the good linearity and a high stability substantially enables this method promising application in physiology and pathology. ► A CMM based dual biosensor was constructed for the first time. ► Pt NPs were assembled on PDDA-functionalized CMM via electrostatic interaction. ► Glucose and L-lactate in microdialysate of rats were simultaneously determined.