Details

Autor(en) / Beteiligte

• Zeppa, Pio
• Sosa Fernandez, Laura Virginia
• Cozzolino, Immacolata
• Ronga, Valentina
• Genesio, Rita
• Salatiello, Maria
• Picardi, Marco
• Malapelle, Umberto
• Troncone, Giancarlo
• Vigliar, Elena

Titel

Immunoglobulin heavy‐chain fluorescence in situ hybridization‐chromogenic in situ hybridization DNA probe split signal in the clonality assessment of lymphoproliferative processes on cytological samples

Ist Teil von

• Cancer cytopathology, 2012-12, Vol.120 (6), p.390-400

Ort / Verlag

Hoboken, USA: John Wiley & Sons, Inc

Erscheinungsjahr

2012

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• BACKGROUND: The human immunoglobulin heavy‐chain (IGH) locus at chromosome 14q32 is frequently involved in different translocations of non‐Hodgkin lymphoma (NHL), and the detection of any breakage involving the IGH locus should identify a B‐cell NHL. The split‐signal IGH fluorescence in situ hybridization‐chromogenic in situ hybridization (FISH‐CISH) DNA probe is a mixture of 2 fluorochrome‐labeled DNAs: a green one that binds the telomeric segment and a red one that binds the centromeric segment, both on the IGH breakpoint. In the current study, the authors tested the capability of the IGH FISH‐CISH DNA probe to detect IGH translocations and diagnose B‐cell lymphoproliferative processes on cytological samples. METHODS: Fifty cytological specimens from cases of lymphoproliferative processes were tested using the split‐signal IGH FISH‐CISH DNA probe and the results were compared with light‐chain assessment by flow cytometry (FC), IGH status was tested by polymerase chain reaction (PCR), and clinicohistological data. RESULTS: The signal score produced comparable results on FISH and CISH analysis and detected 29 positive, 15 negative, and 6 inadequate cases; there were 29 true‐positive cases (66%), 9 true‐negative cases (20%), 6 false‐negative cases (14%), and no false‐positive cases (0%). Comparing the sensitivity of the IGH FISH‐CISH DNA split probe with FC and PCR, the highest sensitivity was obtained by FC, followed by FISH‐CISH and PCR. CONCLUSIONS: The split‐signal IGH FISH‐CISH DNA probe is effective in detecting any translocation involving the IGH locus. This probe can be used on different samples from different B‐cell lymphoproliferative processes, although it is not useful for classifying specific entities. Cancer (Cancer Cytopathol) 2012;. © 2012 American Cancer Society. The split‐signal immunoglobulin heavy‐chain (IGH) fluorescence in situ hybridization‐chromogenic in situ hybridization (FISH‐CISH) DNA probe is effective in the detection of any translocation involving the IGH locus. It can be used on different cytological samples from different B‐cell lymphoproliferative processes, although it is not useful for classifying the specific entities.