Details

Autor(en) / Beteiligte

• Ruthes, Andrea C.
• Rattmann, Yanna D.
• Malquevicz-Paiva, Simone M.
• Carbonero, Elaine R.
• Córdova, Marina M.
• Baggio, Cristiane H.
• Santos, Adair R.S.
• Gorin, Philip A.J.
• Iacomini, Marcello

Titel

Agaricus bisporus fucogalactan: Structural characterization and pharmacological approaches

Ist Teil von

• Carbohydrate polymers, 2013-01, Vol.92 (1), p.184-191

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• ► A fucogalactan (EFP-Ab) from A. bisporus was characterized structurally. ► EFP-Ab inhibited significantly inflammatory pain caused by formalin injection. ► Lethality induced by CLP was decreased by 40%. ► iNOS and COX-2 protein expression were markedly decreased. ► EFP-Ab has significant anti-sepsis, antinociceptive and anti-inflammatory actions. The fucogalactan from Agaricus bisporus (EFP-Ab) obtained on aqueous extraction followed by purification had Mw 37.1×104gmol−1 relative to a (1→6)-linked α-d-Galp main-chain partially methylated at HO-3, and partially substituted at O-2 by nonreducing end-units of α-l-Fucp or β-d-Galp. EFP-Ab also inhibited significantly the neurogenic and inflammatory phases of formalin-induced licking, however, the antinociceptive effect was more pronounced against the inflammatory phase with ID50 of 36.0 (25.8–50.3)mgkg−1. In addition, EFP-Ab decreased the lethality induced by CLP. Its administration reduced the late mortality rate by 40%, prevented neutrophil accumulation in lungs and markedly decreased iNOS and COX-2 protein expression by ileum cells. These data show for the first time that EFP-Ab has significant anti-sepsis, antinociceptive and anti-inflammatory actions, which seems to be related to the decreased iNOS and COX-2 expression. Collectively, the present results demonstrate that EFP-Ab could constitute an attractive molecule of interest for the development of new drugs.