Journal of microbiological methods, 2012-10, Vol.91 (1), p.117-120
2012
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- Autor(en) / Beteiligte
- Titel
- Optimization of the URA-blaster disruption system in Candida guilliermondii: Efficient gene targeting using the URA3 marker
- Ist Teil von
- Journal of microbiological methods, 2012-10, Vol.91 (1), p.117-120
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We established a simple transformation system for C. guilliermondii by developing both an ura3 ATCC 6260-derived recipient strain as well as an URA3 blaster cassette. We demonstrated that this strategy allows efficient multiple gene disruption by homologous recombination with a convenient gene targeting frequency. ► A simple transformation system for C. guilliermondii was established. ► An ATCC 6260-derived uracil auxotroph strain and a URA3 blaster cassette were developed. ► This strategy allows efficient multiple gene disruption by homologous recombination.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0167-7012eISSN: 1872-8359DOI: 10.1016/j.mimet.2012.07.020Titel-ID: cdi_proquest_miscellaneous_1237085676
- Format
- –
- Schlagworte
- Biological and medical sciences, Candida - genetics, Candida guilliermondii, Fluoroorotic acid, Fundamental and applied biological sciences. Psychology, Fungal Proteins - genetics, Fungal Proteins - metabolism, gene targeting, Gene Targeting - methods, homologous recombination, Meyerozyma guilliermondii, Microbiology, Mycological methods and techniques used in mycology, Mycology, Recombination, Genetic, Selectable marker, Selection, Genetic, Transformation system, URA3