Details

Autor(en) / Beteiligte

• Chan, K.H.
• Chan, K.M.
• Ho, Y.L.
• Lam, Y.P.
• Tong, H.L.
• Poon, L.L.M.
• Cowling, B.J.
• Peiris, J.S.M.

Titel

Quantitative analysis of four rapid antigen assays for detection of pandemic H1N1 2009 compared with seasonal H1N1 and H3N2 influenza A viruses on nasopharyngeal aspirates from patients with influenza

Ist Teil von

• Journal of virological methods, 2012-12, Vol.186 (1-2), p.184-188

Ort / Verlag

Kidlington: Elsevier B.V

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• ► It is essential to define diagnostic performance of rapid influenza detection kits. ► The rapid kits had lower sensitivity for diagnosis of H1pdm09 than seasonal flu. ► Direct immunofluorescence test also showed similar finding. ► Lower sensitivity of the rapid kits is related to lower viral load in the patients. ► The detection limit of the rapid kits in clinical specimens was 1×107copies/ml. Data on analytical sensitivity of rapid diagnostic assays are important for clinical management of influenza, especially during a pandemic. Four rapid antigen detection assays were compared for detection of pandemic influenza A H1N1 2009, seasonal H1N1 and H3N2 in 96 patients with influenza A infection confirmed by real-time RT-PCR. These rapid antigen tests appeared to have lower sensitivity (55.8%) for the diagnosis of pandemic influenza A H1N1 2009 as compared with seasonal H3N2 (71.0%) or H1N1 (69.4%) influenza infections, a difference that was related to a lower viral load in patients infected with the pandemic influenza A H1N1 2009 virus. The detection limit of these antigen detection tests in clinical specimens was an influenza A M gene copy number of average 1.0×107copies/ml. Significant variations between tests in sensitivity for detection of pandemic influenza A H1N1 2009 (43.4–63.3%) were observed. The Directigen EZ Influenza A+B and the Espline Influenza A+B had comparable sensitivity (63%) and were the most sensitive among the four assays evaluated.