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Regulatory Infrared Spectroscopic Method for the Rapid Determination of Total Isolated Trans Fat: A Collaborative Study
Ist Teil von
Journal of the American Oil Chemists' Society, 2011-01, Vol.88 (1), p.39-46
Ort / Verlag
Berlin/Heidelberg: Springer-Verlag
Erscheinungsjahr
2011
Link zum Volltext
Quelle
Wiley Blackwell Single Titles
Beschreibungen/Notizen
Using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, collaborating scientists in ten different laboratories measured (in duplicate) the total
trans
fat content of ten fat or oil test samples, two of which were blind duplicates. The procedure used entailed measuring the height of the negative second derivative of the IR absorption band at 966 cm
−1
. This absorption is attributed to the C–H deformation vibration that is characteristic of isolated (non-conjugated) double bonds with the
trans
configuration. The precision of ATR-FTIR results in this international collaborative study was satisfactory and led to the approval of this validated procedure as official method AOCS Cd 14e-09 in late 2009. This official method is also suitable for analysis of total isolated
trans
fat and oil products containing, or supplemented with,
trans
conjugated linoleic acid (CLA) isomers. Although this method does not require derivatization of the oil or fat test materials, as required for GC, fats and oils in foods must be extracted with organic solvents before analysis. This method is also rapid (5 min) and does not require any weighing or quantitative dilution of unknown neat fat or oil test samples in any solvent. The AOCS Cd 14e-09 method is suitable for determination of test samples with zero
trans
fat, which is defined according to the US labeling regulations as 0.5 g
trans
fat per serving or 1.8%
trans
fat, as a percentage of total fat.