Nutrition and cancer, 2012-01, Vol.64 (8), p.1269-1278
2012
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- Autor(en) / Beteiligte
- Titel
- Effects of Tepary Bean (Phaseolus acutifolius) Protease Inhibitor and Semipure Lectin Fractions on Cancer Cells
- Ist Teil von
- Nutrition and cancer, 2012-01, Vol.64 (8), p.1269-1278
- Ort / Verlag
- United States: Taylor & Francis Group
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Some natural and synthetic protease inhibitors (PI), such as the Bowman-Birk PI from soybean, have anticancer effects. We previously purified and characterized a Bowman-Birk-type PI from Tepary bean (Phaseolus acutifolius) seeds (TBPI). A semipure protein fraction containing this inhibitor, when tested its in vitro effect on transformed cells, showed a differential cytotoxic effect, as well as an increase in cell attachment to culture dishes. In this article we report that lectins were responsible for the cytotoxic effect previously observed, exhibiting a differential, antiproliferative effect on nontransformed cells and on different lineages of cancer cells. Although the purified TBPI lacked cytotoxicity, it was found to be responsible for the increase in cell adhesion, decreasing culture dishes’ extracellular matrix degradation, leading to a decrease of the in vitro cell invasion capacity. This effect coincided with the suppression of Matrix Metalloproteinase-9 activity. These results indicate that Tepary bean seeds contain at least 2 different groups of bioactive proteins with distinct effects on cancer cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1532-7914, 0163-5581eISSN: 1532-7914DOI: 10.1080/01635581.2012.722246Titel-ID: cdi_proquest_miscellaneous_1179493074
- Format
- –
- Schlagworte
- 3T3 Cells, Animals, anticarcinogenic activity, Caco-2 Cells, Cancer, cell adhesion, Cell Adhesion - drug effects, cell invasion, Cell Line, Tumor, Cell Proliferation - drug effects, Cells, Cytotoxicity, extracellular matrix, Extracellular Matrix - drug effects, Extracellular Matrix - metabolism, gelatinase A, HeLa Cells, Humans, lectins, Lectins - pharmacology, MCF-7 Cells, Mice, Neoplasm Invasiveness - prevention & control, Phaseolus - chemistry, Phaseolus acutifolius var. acutifolius, Protease inhibitors, Protease Inhibitors - pharmacology, proteinase inhibitors, Proteins, seeds, Seeds - chemistry, soybeans