Journal of virological methods, 2012-10, Vol.185 (1), p.156-159
2012
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Curing viruses in Pleurotus ostreatus by growth on a limited nutrient medium containing cAMP and rifamycin
- Ist Teil von
- Journal of virological methods, 2012-10, Vol.185 (1), p.156-159
- Ort / Verlag
- Kidlington: Elsevier B.V
- Erscheinungsjahr
- 2012
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- Oyster mushroom spherical virus (OMSV) and oyster mushroom isometric virus (OMIV) are the causative agents of a fruiting body deformation disease in the edible mushroom Pleurotus ostreatus. The curing of these mycoviruses was facilitated by a serial transfer of infected mycelia onto a limited nutrient medium containing 1mM of cAMP and 75μg/ml of rifamycin (cAMP-rifamycin plate). The mycelia were grown on cAMP-rifamycin plates for 5 successive passages. ELISA and RT-PCR showed that the amount of mycoviruses inside the mycelia decreased significantly with increasing numbers of passages. The mycelia became free of viruses after 5 successive passages. Cultivation of the virus-cured mycelia on a mushroom compost medium produced a normal harvest, whereas the spawn infected with viruses failed to produce any fruiting bodies.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0166-0934eISSN: 1879-0984DOI: 10.1016/j.jviromet.2012.06.002Titel-ID: cdi_proquest_miscellaneous_1125228732
- Format
- –
- Schlagworte
- Agronomy. Soil science and plant productions, Antiviral Agents - metabolism, Basidiocarps, Biological and medical sciences, cAMP, Composts, Culture Media - chemistry, Curing, Cyclic AMP, Cyclic AMP - metabolism, Edible fungi production, Enzyme-linked immunosorbent assay, Enzyme-Linked Immunosorbent Assay - methods, Fruit bodies, fruiting bodies, Fundamental and applied biological sciences. Psychology, General agronomy. Plant production, Isometric, Microbiology, mushroom compost, mushrooms, Mycelia, mycelium, Mycological methods and techniques used in mycology, Mycology, Mycology - methods, Mycovirus, mycoviruses, Nutrients, Oyster mushroom, Pleurotus - growth & development, Pleurotus - virology, Pleurotus ostreatus, Polymerase chain reaction, reverse transcriptase polymerase chain reaction, Reverse Transcriptase Polymerase Chain Reaction - methods, Rifamycins, Rifamycins - metabolism, Techniques used in virology, Virology, virus curing, Viruses - isolation & purification