Bioscience, biotechnology, and biochemistry, 2012, Vol.76 (8), p.1445-1451
2012
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- Autor(en) / Beteiligte
- Titel
- Dieckol Isolated from Ecklonia cava Protects against High-Glucose Induced Damage to Rat Insulinoma Cells by Reducing Oxidative Stress and Apoptosis
- Ist Teil von
- Bioscience, biotechnology, and biochemistry, 2012, Vol.76 (8), p.1445-1451
- Ort / Verlag
- Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Pancreatic β cells are very sensitive to oxidative stress and this might play an important role in β cell death with diabetes. The protective effect of dieckol, one of the phlorotannin polyphenol compounds purified from Ecklonia cava (E. cava), against high glucose-induced oxidative stress was investigated by using rat insulinoma cells. A high-glucose (30 mM) treatment induced the death of rat insulinoma cells, but dieckol, at a concentration 17.5 or 70 µM, significantly inhibited the high-glucose induced glucotoxicity. Treatment with dieckol also dose-dependently reduced thiobarbituric acid reactive substances (TBARS), the generation of intracellular reactive oxygen species (ROS), and the nitric oxide level increased by a high glucose concentration. In addition, the dieckol treatment increased the activities of antioxidative enzymes including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in high glucose-pretreated rat insulinoma cells. Dieckol protected rat insulinoma cells damage under high glucose conditions. These effects were mediated by suppressing apoptosis and were associated with increased anti-apoptotic Bcl-2 expression, and reduced pro-apoptotic cleaved caspase-3 expression. These findings indicate that dieckol might be useful as a potential pharmaceutical agent to protect against the glucotoxicity caused by hyperglycemia-induced oxidative stress associated with diabetes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0916-8451eISSN: 1347-6947DOI: 10.1271/bbb.120096Titel-ID: cdi_proquest_miscellaneous_1113222874
- Format
- –
- Schlagworte
- Animals, Antioxidants - isolation & purification, Antioxidants - pharmacology, apoptosis, Apoptosis - drug effects, Benzofurans - isolation & purification, Benzofurans - pharmacology, Biological and medical sciences, Caspase 3 - genetics, Caspase 3 - metabolism, Catalase - genetics, Catalase - metabolism, dieckol, Dose-Response Relationship, Drug, Ecklonia cava, Fundamental and applied biological sciences. Psychology, Gene Expression - drug effects, Glucose - adverse effects, Glutathione Peroxidase - genetics, Glutathione Peroxidase - metabolism, high glucose, Insulinoma - metabolism, Insulinoma - pathology, Nitric Oxide - antagonists & inhibitors, Nitric Oxide - biosynthesis, oxidative stress, Oxidative Stress - drug effects, Phaeophyceae - chemistry, Proto-Oncogene Proteins c-bcl-2 - genetics, Proto-Oncogene Proteins c-bcl-2 - metabolism, rat insulinoma cell, Rats, Reactive Oxygen Species - antagonists & inhibitors, Reactive Oxygen Species - metabolism, Superoxide Dismutase - genetics, Superoxide Dismutase - metabolism, Thiobarbituric Acid Reactive Substances - metabolism, Tumor Cells, Cultured