Details

Autor(en) / Beteiligte

• Jiang, Kai
• Toedt, Grischa
• Montenegro Gouveia, Susana
• Davey, Norman E.
• Hua, Shasha
• van der Vaart, Babet
• Grigoriev, Ilya
• Larsen, Jesper
• Pedersen, Lotte B.
• Bezstarosti, Karel
• Lince-Faria, Mariana
• Demmers, Jeroen
• Steinmetz, Michel O.
• Gibson, Toby J.
• Akhmanova, Anna

Titel

A Proteome-wide Screen for Mammalian SxIP Motif-Containing Microtubule Plus-End Tracking Proteins

Ist Teil von

• Current biology, 2012-10, Vol.22 (19), p.1800-1807

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2012

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Microtubule plus-end tracking proteins (+TIPs) are structurally and functionally diverse factors that accumulate at the growing microtubule plus-ends, connect them to various cellular structures, and control microtubule dynamics [1, 2]. EB1 and its homologs are +TIPs that can autonomously recognize growing microtubule ends and recruit to them a variety of other proteins. Numerous +TIPs bind to end binding (EB) proteins through natively unstructured basic and serine-rich polypeptide regions containing a core SxIP motif (serine-any amino acid-isoleucine-proline) [3]. The SxIP consensus sequence is short, and the surrounding sequences show high variability, raising the possibility that undiscovered SxIP containing +TIPs are encoded in mammalian genomes. Here, we performed a proteome-wide search for mammalian SxIP-containing +TIPs by combining biochemical and bioinformatics approaches. We have identified a set of previously uncharacterized EB partners that have the capacity to accumulate at the growing microtubule ends, including protein kinases, a small GTPase, centriole-, membrane-, and actin-associated proteins. We show that one of the newly identified +TIPs, CEP104, interacts with CP110 and CEP97 at the centriole and is required for ciliogenesis. Our study reveals the complexity of the mammalian +TIP interactome and provides a basis for investigating the molecular crosstalk between microtubule ends and other cellular structures. ► Combining proteomics with bioinformatics is an effective way of +TIP identification ► SxIP motif-containing EB-binding +TIPs are numerous in mammalian genomes ► CEP104 is an EB-binding +TIP involved in ciliogenesis ► AMER2 reveals microtubule tip-plasma membrane contacts