Biomedical chromatography, 2012-11, Vol.26 (11), p.1408-1415
2012
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- Autor(en) / Beteiligte
- Titel
- Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach
- Ist Teil von
- Biomedical chromatography, 2012-11, Vol.26 (11), p.1408-1415
- Ort / Verlag
- Chichester, UK: John Wiley & Sons, Ltd
- Erscheinungsjahr
- 2012
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- ABSTRACT The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high‐performance liquid chromatography (RP‐HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 ‐ 350 mm of lactate, 0.003‐ 1 mm of pyruvate. The sensitivity was good with a limit of detection of 0.03 mm for lactate and 0.001 mm for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise. Copyright © 2012 John Wiley & Sons, Ltd.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0269-3879eISSN: 1099-0801DOI: 10.1002/bmc.2713Titel-ID: cdi_proquest_miscellaneous_1082407085
- Format
- –
- Schlagworte
- Adult, Chromatography, High Pressure Liquid - methods, Chromatography, Reverse-Phase - methods, Exercise, Female, Humans, lactate, lactate/pyruvate ratio, Lactic Acid - analysis, Lactic Acid - metabolism, Linear Models, Male, pyruvate, Pyruvic Acid - analysis, Pyruvic Acid - metabolism, Reproducibility of Results, Rest, reversed-phase chromatography, Sensitivity and Specificity, Sweat - chemistry, Sweat - metabolism, sweat analysis