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Cdc42 GTPase and Rac1 GTPase act downstream of p120 catenin and require GTP exchange during gastrulation of zebrafish mesoderm
Developmental dynamics, 2012-10, Vol.241 (10), p.1545-1561
Hsu, Cynthia L.
Muerdter, Claire P.
Knickerbocker, Abhay D.
Walsh, Ryan M.
Zepeda-Rivera, Martha A.
Depner, Kevin H.
Sangesland, Maya
Cisneros, Trinidad B.
Kim, Ju Youn
Sanchez-Vazquez, Patricia
Cherezova, Lidia
Regan, Rainy D.
Bahrami, Nadia M.
Gray, Elizabeth A.
Chan, Andrew Y.
Chen, Terry
Rao, Milly Y.
Hille, Merrill B.
2012
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Hsu, Cynthia L.
Muerdter, Claire P.
Knickerbocker, Abhay D.
Walsh, Ryan M.
Zepeda-Rivera, Martha A.
Depner, Kevin H.
Sangesland, Maya
Cisneros, Trinidad B.
Kim, Ju Youn
Sanchez-Vazquez, Patricia
Cherezova, Lidia
Regan, Rainy D.
Bahrami, Nadia M.
Gray, Elizabeth A.
Chan, Andrew Y.
Chen, Terry
Rao, Milly Y.
Hille, Merrill B.
Titel
Cdc42 GTPase and Rac1 GTPase act downstream of p120 catenin and require GTP exchange during gastrulation of zebrafish mesoderm
Ist Teil von
Developmental dynamics, 2012-10, Vol.241 (10), p.1545-1561
Ort / Verlag
New York: Wiley-Liss, Inc
Erscheinungsjahr
2012
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
Background: We investigated the roles of p120 catenin, Cdc42, Rac1, and RhoA GTPases in regulating migration of presomitic mesoderm cells in zebrafish embryos. p120 catenin has dual roles: It binds the intracellular and juxtamembrane region of cadherins to stabilize cadherin‐mediated adhesion with the aid of RhoA GTPase, and it activates Cdc42 GTPase and Rac1 GTPase in the cytosol to initiate cell motility. Results: During gastrulation of zebrafish embryos, knockdown of the synthesis of zygotic p120 catenin δ1 mRNAs with a splice‐site morpholino caused lateral widening and anterior‐posterior shortening of the presomitic mesoderm and somites and a shortened anterior‐posterior axis. These phenotypes indicate a cell‐migration effect. Co‐injection of low amounts of wild‐type Cdc42 or wild‐type Rac1 or dominant‐negative RhoA mRNAs, but not constitutively‐active Cdc42 mRNA, rescued these p120 catenin δ1‐depleted embryos. Conclusions: These downstream small GTPases require appropriate spatiotemporal stimulation or cycling of GTP to guide mesodermal cell migration. A delicate balance of Rho GTPases and p120 catenin underlies normal development. Developmental Dynamics 241:1545–1561, 2012. © 2012 Wiley Periodicals Inc. Key findings p120 catetin is required for extension of the dorsal axis and normal migration of the presomitic mesoderm. Cdc42 and Rac1 GTPases are downstream of p120 catenin δ1 signaling and require exchange of GTP for GDP. Local stimulation of the exchange of GTP for GDP in Cdc42 and Rac GTPases mediates directional migration of the presomitic mesoderm. A balance of the amount of p120 catenin δ1 and localized activation or turnover of Cdc42, Rac1, and Rho GTPase are required for normal zebrafish cell migration.
Sprache
Englisch
Identifikatoren
ISSN: 1058-8388
eISSN: 1097-0177
DOI: 10.1002/dvdy.23847
Titel-ID: cdi_proquest_miscellaneous_1080882940
Format
–
Schlagworte
Animals
,
ARVCF
,
Blotting, Western
,
Catenins - genetics
,
Catenins - metabolism
,
cdc42 GTP-Binding Protein - metabolism
,
Cdc42 GTPase
,
Cell Movement - physiology
,
Cloning, Molecular
,
Delta-catenin (CTNND2b)
,
gastrulation
,
Gastrulation - physiology
,
Gene Knockdown Techniques
,
Guanosine Triphosphate - metabolism
,
In Situ Hybridization
,
Mesoderm - cytology
,
Mesoderm - enzymology
,
Mesoderm - metabolism
,
Oligonucleotides, Antisense - genetics
,
p120 Catenin (CTNND1)
,
presomitic mesoderm
,
rac1 GTP-Binding Protein - metabolism
,
Rac1 GTPase
,
Rac1 GTPase, RhoA GTPase
,
Real-Time Polymerase Chain Reaction
,
Reverse Transcriptase Polymerase Chain Reaction
,
RhoA GTPase
,
somites
,
zebrafish
,
Zebrafish - embryology
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