Details

Autor(en) / Beteiligte

• Mascré, Guilhem
• Dekoninck, Sophie
• Drogat, Benjamin
• Youssef, Khalil Kass
• Brohée, Sylvain
• Sotiropoulou, Panagiota A.
• Simons, Benjamin D.
• Blanpain, Cédric

Titel

Distinct contribution of stem and progenitor cells to epidermal maintenance

Ist Teil von

• Nature (London), 2012-09, Vol.489 (7415), p.257-262

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2012

Quelle

EBSCOhost Psychology and Behavioral Sciences Collection

Beschreibungen/Notizen

• The skin interfollicular epidermis (IFE) is the first barrier against the external environment and its maintenance is critical for survival. Two seemingly opposite theories have been proposed to explain IFE homeostasis. One posits that IFE is maintained by long-lived slow-cycling stem cells that give rise to transit-amplifying cell progeny, whereas the other suggests that homeostasis is achieved by a single committed progenitor population that balances stochastic fate. Here we probe the cellular heterogeneity within the IFE using two different inducible Cre recombinase–oestrogen receptor constructs targeting IFE progenitors in mice. Quantitative analysis of clonal fate data and proliferation dynamics demonstrate the existence of two distinct proliferative cell compartments arranged in a hierarchy involving slow-cycling stem cells and committed progenitor cells. After wounding, only stem cells contribute substantially to the repair and long-term regeneration of the tissue, whereas committed progenitor cells make a limited contribution. Whether a single group of stem cells or multiple populations contribute to the homeostasis of the interfollicular epidermis is controversial; here the authors use lineage tracing and mathematical modelling to show that the progenitors that maintain mouse epidermis are underpinned by slow-cycling stem cells that become mobilized on injury. Twin-track approach to epidermal-cell renewal Skin epidermis consists of a basal layer of proliferative cells and several suprabasal layers of terminally differentiated cells that are progressively enucleated and shed from the skin surface. The cells that maintain this important barrier to infection and injury are generated in the interfollicular epidermis, but whether this involves a single group of stem cells or multiple populations is controversial. Cédric Blanpain and colleagues use lineage tracing and mathematical modelling to show that there are two classes of stem cell in mouse tail skin: a previously reported population of committed progenitors, and slow-cycling stem cells that divide asymmetrically only about four to six times a year. During homeostasis, asymmetric divisions of slow-cycling and committed progenitors give rise to transient amplifying cells and differentiated cells, respectively. But during wound healing, the slow-cycling stem cells make a significantly larger and more sustained contribution to tissue repair and regeneration.